Microbiological Diagnostic Unit, Public Health Laboratory - University of Melbourne

Site

The University of Melbourne – At the Peter Doherty Institute for Infection & Immunity

Accreditation No.

1019

Site No.

16879

Date of Accreditation

05 Jan 2009

Address

Department of Microbiology and Immunology, At the Peter Doherty Institute for Infection and Immunity, Level 1, 792 Elizabeth St (cnr of Grattan and Elizabeth St)
Melbourne, VIC 3000
Australia

mduphl.unimelb.edu.au

Contact

Ms Selena Heaton P: +61 (03) 83445701

Availability

Services available to external clients

Supervision

Scientist Supervised Laboratory

Scope of Accreditation

The University of Melbourne – At the Peter Doherty Institute for Infection & Immunity

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Antibiotic sensitivity (susceptibility) testing Bacterial isolates; Fungal isolates Zone of growth inhibition Agar dilution method in-house method ENS053
Detection and identification of bacteria Bacterial isolates Bacillus anthracis; Burkholderia pseudomallei; Campylobacter spp.; Clostridium botulinum; Escherichia coli - Enterotoxigenic (ETEC); Escherichia coli - Shiga toxin-producing (STEC); Escherichia coli​​​​; Escherichia coli​​​​ O111; Escherichia coli​​​​ O157; Escherichia coli​​​​ O26; Escherichia coli​​​​ uspA; Escherichia coli​​​​ virulence factors; Listeria monocytogenes; Listeria monocytogenes binary typing; Salmonella spp.; Shigella spp.; Staphylococcus aureus; Staphylococcus aureus (including MRSA) Panton-Valentine leukcocidin (PVL) gene; Staphylococcus aureus (including MRSA) lukF gene; Staphylococcus aureus (including MRSA) lukS gene; Yersinia spp. Polymerase chain reaction (PCR) in-house methods MMS058, MMS078, MMS022, MMS034, MMS035, MMS084, MMS015, MMS036, MMS037 and MMS085
Bacillus anthracis; Bacillus cereus; Brucella spp.; Burkholderia pseudomallei; Campylobacter coli; Campylobacter fetus ss. fetus; Campylobacter fetus ss. venerealis (Cfv); Campylobacter fetus; Campylobacter hyointestinalis; Campylobacter jejuni; Campylobacter spp.; Clostridiodes difficile; Clostridium botulinum; Clostridium difficile toxin B; Corynebacterium spp.; Escherichia coli serotyping; Escherichia coli K99; Escherichia coli​​​​; Escherichia coli​​​​ - Haemolytic; Escherichia coli​​​​ - Non-haemolytic; Francisella tularensis; Nocardia spp.; Rhodococcus equi; Salmonella enterica serovar typhimurium; Salmonella pullorum; Salmonella spp.; Salmonella typhimurium; Salmonella serotype; Vibrio alginolyticus; Vibrio cholerae; Vibrio furnissii; Vibrio harveyi; Vibrio jasicida; Vibrio mimicus; Vibrio ordalii; Vibrio owensii; Vibrio parahaemolyticus; Vibrio penaeicida; Vibrio proteolyticus; Vibrio rotiferianus; Vibrio salmonicida; Vibrio splendidus; Vibrio spp.; Vibrio tapetis; Vibrio tasmaniaensis; Vibrio tubiashii; Yersinia enterocolitica; Yersinia intermedia; Yersinia kristensenii; Yersinia pseudotuberculosis; Yersinia ruckeri; Yersinia spp.; Aerobic spore forming gram positive bacilli; Identification of unknown bacteria Biochemical; Cultural; Microscopic examination in-house methods CAS002, CRS001, ECS001, ENS003, ENS032, ENS034, RFS002, RFS017, RFS018, RFS019, RFS021, RFS022, RFS023 and RFS024
Identification of unknown bacteria Sequencing in-house method MMS012
Salmonella enteritidis; Salmonella hadar; Salmonella paratyphi; Salmonella typhimurium; Salmonella vichow Serotyping; Serum agglutination in-house methods ENS005 and ENS010
Identification of unknown bacteria MALDI-TOF in-house method MSS001 software version V3.2
Enrichment broth; Faeces Escherichia coli​​​​ stx gene Polymerase chain reaction (PCR) in-house method MMS035
Faeces Actinomyces pyogenes; Aeromonas spp.; Bacillus anthracis; Bacillus cereus; Campylobacter coli; Campylobacter fetus ss. fetus; Campylobacter fetus ss. venerealis (Cfv); Campylobacter fetus; Campylobacter hyointestinalis; Campylobacter jejuni; Campylobacter spp.; Clostridiodes difficile; Clostridium difficile toxin B; Clostridium perfringens; Escherichia coli - Shiga toxin-producing (STEC); Escherichia spp.; Listeria monocytogenes; Listeria spp.; Plesiomonas spp.; Salmonella enterica serovar typhimurium; Salmonella pullorum; Salmonella spp.; Salmonella typhimurium; Salmonella serotype; Shigella spp.; Staphylococcus aureus; Vibrio cholerae; Vibrio spp.; Yersinia enterocolitica; Coliforms; Enteric bacteria; Identification of unknown bacteria Biochemical; Cultural; Microscopic examination; Staining in-house methods BFS002, BFS005, BFS006, BFS007, BFS008, BFS010, BFS011, BFS012, BFS013, BFS014, BFS015, BFS019, BFS026, BFS027, BFS050, BFS051, BFS052 and BFS053
Detection and identification of parasites and arthropods Faeces Cryptosporidium spp.; Giardia spp. Immunochromatography in-house method BFS022 (RIDA®QUICK Cryptosporidium/Giardia Combi Test immunochromatographic rapid assay)
Cryptosporidium hominis; Cryptosporidium parvum; Cryptosporidium spp. Polymerase chain reaction (PCR) in-house method MMS023
Detection and identification of viruses Faeces Adenovirus; Rotavirus Cultural in-house method BFS025
Legal management - Forensic operations module Materials defined by all or some for which the site is accredited Case record review; Chain of custody; Control of records; Court testimony monitoring; Sample/evidence integrity; Security and access
Molecular analysis - Bioinformatic analysis and interpretation Bacterial isolates Single nucleotide polymorphism (SNP) interpretation Bioinformatics pipeline In-house method MMS147
Molecular analysis - Extraction Bacterial isolates Viable DNA for sequencing Pulse field gel electrophoresis in-house method MMS006
Molecular analysis - Sequencing Bacterial isolates Genotyping by fragment separation Polymerase chain reaction (PCR) in-house method MMS057 - Salmonella method (MLVA)
Single nucleotide polymorphism (SNP) detection Whole genome sequencing In-house method MMS147
Massively parallel sequencing (MPS) Massively parallel sequencing (MPS) in-house method MMS103
Antimicrobial resistance genes Whole genome sequencing In-house method MMS118
Escherichia coli serotyping Whole genome sequencing In-house method MMS130
Allele typing; Sanger sequencing Multilocus sequence typing (MLST) in-house method MMS109
Sanger sequencing Sanger sequencing in-house methods MMS012 and MMS023
Salmonella spp. serotyping Whole genome sequencing In-house method MMS136

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