Biosecurity Queensland Veterinary Laboratories - Department of Agriculture and Fisheries

Site

Biosecurity Sciences Laboratory

Accreditation No.

13389

Site No.

13382

Date of Accreditation

11 Jan 2000

Address

Specimen Receipt (Loading Dock 12), Health and Food Sciences Precinct, 39 Kessels Road
Coopers Plains, QLD 4108
Australia

business.qld.gov.au/industries/farms-fishing-forestry/agriculture/biosecurity/lab/services

Contact

Dr Louise Jackson P: +61 (07) 37088762

Availability

Services available to external clients

Supervision

Veterinarian Supervised Laboratory

Scope of Accreditation

BSL – Anatomical and Aquatic Pathology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Examination of biopsy material Tissues Prion disease lesions Histopathology; Microscopic examination

Australian and New Zealand Standard Diagnostic Procedure, Transmissible Spongiform Encephalopathies, August 2010; in-house method PATH-002.

Histological examination Histopathology; Macroscopic examination; Microscopic examination; Special stains

in-house methods HIS-001 (tissue processing); PATH-002 (histological interpretation); HIS-008 (Congo Red); HIS-009 (Feulgen Reaction); HIS-010 (Giemsa ARI); HIS-011 (Giemsa); HIS-047 (DIFF Quick); HIS-013 (Gram); HIS-014 (Grocott's Methamine Silver); HIS-017 (Luxol Fast Blue); HIS-020 (Haematoxylin and eosin-phloxine); HIS-015 (Hall's method-Fouchet's technique); HIS-017 (Luxol Fast Blue); HIS-018 (Mallory Phosphotungstic Acid Haematoxylin); HIS-019 (Masson Trichrome); HIS-021 (Alcian Blue Periodic Acid Schiff's Technique); HIS-022 (Periodic Acid Schiff's Technique); HIS-023 (PAS and Diastase); HIS-024 (Perl's Prussion Blue);  HIS-025 (Phloxine Tartrazine); HIS-026 (Schmorl's Reaction); HIS-029 (Toluidine Blue); HIS-030 (Von-Kossa Technique); HIS-031 (Ziehl Neelsen); HIS-035 (Warthin-Starry); HIS-045 (Bielshowsky Silver Luxol fast Blue Staining); HIS-050 (Wade-Fite); HIS-051 (Rhodanine method for Copper); HIS-052 (Okajima Stain for Haemoglobin); HIS-053 (VVG -Verhoeff Van Gieson for elastic fibres); HIS – 055 (Reticular Fibre Staining technique); HIS-056 (Gimenez Stain).




Necropsy servicesFor production animals, production avian species, wildlife, aquatic animals, equine species and avian species Body; Tissues Gross pathology and collection of sub-samples Anatomical pathology; Microscopic examination

in-house method PATH-001

BSL – Genetics

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Molecular analysis - Bioinformatic analysis and interpretation DNA sequences Amplicon identity Comparison of aligned sequences to database of reference sequences in house method GA-001
Molecular analysis - SequencingFor aquatic animals, avian species, bees/apiculture, companion animals, equine species, laboratory animals, production animals, production avian species, wildlife, zoo animals. PCR product Nucleotide sequencing Sanger sequencing in house method GA-001 (3500xL)
BSL – Molecular

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Detection and identification of bacteriaFor avian species Conjunctival swabs; Sinus swabs; Swabs; Tracheal swabs Avibacterium paragallinarum PCR - Quantitative (qPCR)

in-house method MOL-028 

Larynx swabs; Liver; Lung; Oropharynx; Swabs; Tissues; Tracheal swabs Pasteurella multocida PCR - Quantitative (qPCR) - Reverse transcription in-house method MOL-027
Detection and identification of bacteriaFor production animals Kidney; Liver; Tissues; Urine Leptospira spp. PCR - Quantitative (qPCR)

in-house method MOL-042

Detection and identification of parasites and arthropodsFor production animals Preputial smegma; Swabs; Vaginal secretions Tritrichomonas foetus PCR - Quantitative (qPCR)

in-house method MOL-043

Detection and identification of viruses Colon; Faeces; Ileum; Rectal swabs Rotavirus PCR - Quantitative (qPCR) in-house method MOL-058
Culture materials; Kidney; Liver; Lung; Spleen; Swabs; Tissues Equine herpes virus type 1 PCR - Quantitative (qPCR) in-house method MOL-018 (EHV Type 1 only)
Detection and identification of virusesFor aquatic animals (prawns, crabs) Gills; Pleopod; Tissues White spot syndrome virus (WSSV) PCR - Quantitative (qPCR) - Reverse transcription in-house method MOL-052
Detection and identification of virusesFor avian species Cloacal swabs; Kidney; Liver; Lung; Pancreas; Spleen; Swabs; Tissues; Tracheal swabs Avian paramyxovirus 1 PCR - Quantitative (qPCR)

in-house methods MOL-022 and MOL-024

Detection and identification of virusesFor equine species Culture materials; Kidney; Liver; Lung; Spleen; Swabs; Tissues Equine herpes virus; Equine herpes virus type 4 PCR - Quantitative (qPCR) - Multiplex

in-house method MOL-032 (EHV Type 1 & 4 only)

Kidney; Liver; Lung; Pancreas; Spleen; Swabs; Tissues; Tracheal swabs Influenza virus PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-023 (Influenza Type A)

Nasal swabs; Oral swabs; Rectal swabs; Serum; Swabs; Urethral swabs; Urine; Vaginal swabs; Whole blood Hendra virus PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-033

Detection and identification of virusesFor production animals (porcine) Brain tissue; Cerebrospinal fluid (CSF); Meninges; Placenta Japanese encephalitis virus (JEV) PCR - Quantitative (qPCR) in-house method MOL-059
Detection and identification of virusesFor production animals Brain tissue; Culture materials; Lung; Semen; Swabs; Tissues; Trachea Bovine herpes virus type 1; Bovine herpes virus type 5 PCR - Quantitative (qPCR)

in-house method MOL-036

Buffy coat; Culture materials; Liver; Spleen; Swabs; Tissues Bovine viral diarrhoea virus (BVDV) PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-021

Buffy coat; Erythrocytes (red blood cells, RBC); PCR product Bovine immunodeficiency virus; Bovine leukaemia virus; Bovine spumavirus PCR - Quantitative (qPCR); PCR - Quantitative (qPCR) - Multiplex

in-house methods MOL-039; MOL-014

Whole blood Bluetongue virus (BTV) PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-050 

Bovine ephemeral fever virus PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-029

Detection and identification of virusesFor production avian species, avian species and wildlife Culture materials; Larynx; Lung; Oropharynx; Swabs; Tissues; Trachea Infectious laryngotracheitis virus PCR - Quantitative (qPCR)

in-house method MOL-026

Detection and identification of virusesFor wildlife (bats) Brain tissue; Tissues Australian bat lyssavirus (ABLV) PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-038

ISO/IEC 17025 (2017)

Healthcare, Pharmaceutical and Media Products

Service Product Determinant Technique Procedure
Testing for sterility and freedom from contamination of biological materials intended for veterinary use - Innocuity Raw materials of animal origin - Vaccines Bovine herpes virus; Bovine viral diarrhoea virus (BVDV) PCR - Quantitative (qPCR) in-house methods MOL-021 (BVD) and MOL-020 (BoHV-1) 
BSL – Parasitology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Analysis of non-clinical samples for restricted animal material Stockfeeds Blood meal; Fish meal; Meat and bone meal; Meat meal; Poultry meal Microscopic examination

in-house method PAR-039; Australian Ruminant Feed Ban National Uniform Guidelines 2021-22

Detection and identification of parasites and arthropodsFor production animals, production avian species, avian species, wildlife, equine species, companion animals, laboratory animals and zoo animals Faeces; Gastrointestinal tract specimens; Intestinal contents; Tissues Coccidia; Strongyles; Helminths Microscopic examination; Morphological identification

in-house methods PAR-001; PAR-004

Fresh or preserved parasites Culicoides spp.; Acarina; External bee mites; Identification of unknown parasites; Insects; Screw worm fly; Tracheal bee mites Microscopic examination

in-house methods PAR-031; PAR-003; PAR-027; PAR-002; PAR-032; PAR;027

Arthropods Resistance assays

in-house methods PAR-016 (Larval Packet Test) and PAR-029 (Adult Immersion Test)

Muscle Trichinella spp. Microscopic examination

in-house method PAR-013

Detection and identification of parasites and arthropodsFor production animals Faeces Liver fluke; Stomach fluke Faecal floatation; Sedimentation

In-house method PAR-010 (Breza and Corbe)

Ascaris spp. (roundworm); Capillaria spp.; Coccidia oocyst count; Nematodirus egg count; Strongyles; Strongylid egg count; Strongyloides egg count; Trichirus (whipworm); Cestoda (Cestoidea) Microscopic examination

in-house method PAR-008 (McMaster)

Lungworm larvae Baermann technique

in-house method PAR-009 and ASDT

Bunostomum larvae; Chabertia larvae; Cooperia larvae; Cyathostomin larvae; Haemonchus larvae; Hyostrongylus larvae; Oesophagostomum larvae; Ostertagia larvae; Strongyloides larvae; Strongylus larvae; Teladorsia larvae; Trichostrongylus larvae; Triodontophorus larvae; Nematodes Cultural; Microscopic examination; Staining

In-house method PAR-011 (larval differentiation)

Faeces; Intestinal contents Coccidia; Cryptosporidium spp.; Helminths Concentration; Faecal floatation

in-house methods PAR-01 and PAR-004 (Anderson method) 

Gastrointestinal tract specimens Identification of unknown parasites Microscopic examination

In-house method PAR-012 (total parasite count)

BSL – Serology and Virology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Detection and identification of virusesFor aquatic animals Plasma; Serum; Swabs; Tissues; Whole blood Aquatic virus; Identification of unknown viruses Cell culture

in-house method VIR-013 and OIE aquatic manual 2009

Detection and identification of virusesFor avian species and production avian species Culture materials Avian paramyxovirus 1 Haemagglutination inhibition

in-house method SER-031, ASDT

Detection and identification of virusesFor production animals and avian species Buffy coat; Plasma; Semen; Serum; Swabs; Tissues; Whole blood Identification of unknown viruses Cell culture

in-house method VIR-002 (ASDT and/or OIE)

Detection and identification of virusesFor production animals and wildlife (bats) Smears; Tissue imprint; Tissues Australian bat lyssavirus (ABLV) Indirect fluorescent antibody

in-house method SER-035 using commercial monoclonal Ab

Detection and identification of virusesFor production animals, production avian species, avian species, wildlife, equine specieS Plasma; Serum; Swabs; Tissues; Whole blood Identification of unknown viruses Embryonated egg culture

in-house method VIR-001 - allantoic and CAM innoculation

Detection and identification of virusesFor production animals Lymph nodes; Spleen; Tissues; Whole blood Bluetongue virus (BTV) Cultural

in-house methods VIR-002 (culture for serotyping and confirmation)

Plasma; Serum; Swabs; Tissues; Whole blood Arbovirus Cell culture

in-house method VIR-012 (ASDT 1993)

Immunohistochemistry investigationFor aquatic animals Tissues Nervous necrosis virus (NNV, Betanodovirus) Paraffin section

in-house method SER-060

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitation Plasma; Serum Ehrlichia canis Enzyme linked immunosorbent assay (ELISA) in-house method SER-090, manufacturers instructions (EUROIMMUN test kit)
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor equine species Plasma; Serum Hendra virus soluble G Enzyme linked immunosorbent assay (ELISA) in-house methods SER-069 and SER-024
Equine infectious anaemia virus Agar gel immunodiffusion (AGID) in-house method SER-004, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT) Equine Infectious Anaemia. 1993.
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine and caprine) Plasma; Serum Mycobacterium paratuberculosis (Johne’s disease) - Bovine; Mycobacterium paratuberculosis (Johne’s disease) - Caprine Enzyme linked immunosorbent assay (ELISA) in-house methods SER-025 and SER-025b; manufacturers instructions (Parachek®2 and IDEXX test kits)
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine) Plasma; Serum Enzootic bovine leukosis virus Enzyme linked immunosorbent assay (ELISA) in-house method SER-092, manufacturers instructions (IDVet IDScreen test kit)
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, companion animals, equine species Plasma; Serum Burkholderia pseudomallei Complement fixation test (CFT) in-house method SER-014 
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, production avian species, wildlife, equine species and avian species Plasma; Serum Burkholderia pseudomalleiAvian paramyxovirus 1 Haemagglutination inhibition

in-house methods SER-033 and SER-031

Serum Leptospira hardjo antibody; Leptospira pomona antibody; Leptospira trassovi antibody Microscopic agglutination

in-house method SER-039 (ASDT)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, wildlife and companion animals (canine) Plasma; Serum Brucella abortus antibody; Brucella suis antibody Rose Bengal plate

in-house method SER-040 (ASDT)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals Plasma; Serum Bovine viral diarrhoea virus (BVDV) Agar gel immunodiffusion (AGID) in-house method SER-007, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Bovine Pestivirus Infections Virology and Serology, 1993.
Brucella ovis Complement fixation test (CFT) in-house method SER-009, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Ovine Brucellosis: Bacteriology and Serology, 1993. 
Enzootic bovine leukosis virus Agar gel immunodiffusion (AGID) in-house method SER-002, Australia and New Zealand Standard Diagnostic Procedures (ANZSDP), Enzootic Bovine Leukosis, 2005.
Brucella abortus; Brucella suis antibody Complement fixation test (CFT) in-house method SER-008, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Bovine Brucellosis: Serology, 1993.
Caprine arthritis encephalitis virus Enzyme linked immunosorbent assay (ELISA) in-house method SER-096 manufacturer's instruction (ID VET ID Screen MVV/CAEV Indirect ELISA) 
Aino virus Serum neutralisation

in-house methods SER-052, Australian and New Zealand Standard Diagnostic Procedures (ANZSDP), Aino Virus, 2001. .

Bovine viral diarrhoea virus (BVDV) Enzyme linked immunosorbent assay (ELISA) in-house method SER-026 (Pestivirus antigen capture), manufacturers instructions (IDEXX kit)
Bluetongue virus (BTV) Agar gel immunodiffusion (AGID) in-house method SER-001, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Bluetongue Virology and Serology, 1993, and manufacturers instructions - VMRD.
Bluetongue virus (BTV) Enzyme linked immunosorbent assay (ELISA)

in-house methods SER-072 and SER-072a, manufacturers instructions (IDEXX and IDVet kits)








Actinobacillus seminis antibody Complement fixation test (CFT)

in-house method SER-011

Neospora caninum antibody Enzyme linked immunosorbent assay (ELISA) in-house method SER-068, manufacturers instructions (IDEXX kit)
Serum Bovine viral diarrhoea virus (BVDV) Serum neutralisation in-house method SER-049, Australia and New Zealand Standard Diagnostic Procedure (ANZSDP), Ruminent Pestivirus Infections, 2006.
Bovine rhinotracheitis virus Serum neutralisation in-house method SER-048, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Infectious Bovine Rhinotracheitis, 1993.
Akabane virus Serum neutralisation in-house method SER-051, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Akabane Disease: Histopathology, Virology and Serology, 1993.
Bovine ephemeral fever virus Serum neutralisation in-house method SER-050
Encephalomyelitis virus Serum neutralisation in-house method SER-053
Swabs in transport media Campylobacter spp. Enzyme linked immunosorbent assay (ELISA) in-house method SER-073

ISO/IEC 17025 (2017)

Healthcare, Pharmaceutical and Media Products

Service Product Determinant Technique Procedure
Testing for sterility and freedom from contamination of biological materials intended for veterinary use - Innocuity Raw materials of animal origin - Vaccines Bovine herpes virus; Infectious bovine rhinotracheitis virus Virus titration - Quantitative in-house method VIR-008; FORM VIR-024A and 024C
BSL-Bacteriology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Analysis of non-clinical samples for microorganisms Animal feeds; Waters Clostridium botulinum toxin Enzyme linked immunosorbent assay (ELISA)

in-house method MICRO-400

Waters Blue green algae (Cyanobacteria) Microscopic examination

in-house method MICRO-404

Antibiotic sensitivity (susceptibility) testing Bacterial isolates Zone of growth inhibition Disc diffusion

in-house method MICRO-003; Clinical and Laboratory Standards Institute (CLSI) method

Detection and identification of bacteria Bacterial isolates Identification of unknown bacteria MALDI-TOF in-house method MICRO-004 using Bruker Library Version V11.0.0.0 Revision H and in-house method MICRO-007 using custom in-house library
Faeces Escherichia coli - Diffusely adherent (DAEC); Escherichia coli - Enteroaggregative (EAEC); Escherichia coli - Enteroinvasive (EIEC); Escherichia coli - Enteropathogenic (EPEC); Escherichia coli - Enterotoxigenic (ETEC); Escherichia coli - Shiga toxin-producing (STEC); Escherichia coli​​​​ virulence factors PCR - Multiplex; Polymerase chain reaction (PCR) in-house method MICRO-602
Detection and identification of bacteriaFor aquatic animals Fluids; Swabs; Tissues Actinobaculum suis (Eubacterium suis); Alcaligenes spp.; Alteromonas piscicida; Alteromonas rubra; Alteromonas spp.; Bacillus mycoides; Bacillus spp.; Bacillus thuringiensis; Carnobacterium piscicola (Carnobacterium maltaromaticum, Lactobacillus piscicola, Lactobacillus maltaromaticum); Carnobacterium spp.; Corynebacterium aquaticum; Corynebacterium spp.; Edwardsiella ictaluri; Edwardsiella spp.; Edwardsiella tarda; Enterococcus faecalis; Enterococcus hirae; Enterococcus spp.; Flavobacteriacae spp.; Flavobacterium spp.; Flexibacter spp.; Lactococcus garvieae; Lactococcus spp.; Mycobacterium chelonae; Mycobacterium marinum; Mycobacterium spp.; Nocardia spp.; Plesiomonas spp.; Pseudomonas anguilliseptica; Pseudomonas fluorescens; Pseudomonas putida; Pseudomonas spp.; Renibacterium salmoninarum; Streptococcus agalactiae (Streptococcus group B); Streptococcus iniae; Streptococcus spp.; Vibrio spp.; Vibrionaceae; Yersinia ruckeri; Yersinia spp.; Identification of unknown bacteria Biochemical; Cultural; Microscopic examination; Staining in-house methods MICRO-200, MICRO-201, MICRO-202,  MICRO-203, MICRO-205 and MEDIA-002.

Detection and identification of bacteriaFor bees/apiculture Brood comb Melissococcus plutonius; Paenibacillus alvei; Paenibacillus larvae Microscopic examination; Staining

in-house methods MICRO-700 and MICRO-701

Honey Paenibacillus larvae Cultural; Microscopic examination

in-house methods MICRO-700 and MICRO-701

Detection and identification of bacteriaFor production animals, production avian species, avian species, wildlife, aquatic animals, equine species, companion animals, laboratory animals, zoo animals and bees/apiculture Scrapings; Skin; Wounds Dermatophilus spp. Biochemical; Cultural; Microscopic examination; Staining

in-house method MICRO-300

Detection and identification of bacteriaFor production animals, production avian species, wildlife, equine species, companion animals, laboratory animals, zoo animals and bees/apiculture Faeces; Fluids; Swabs; Tissues Actinomyces spp.; Bacteroides spp.; Brachyspira spp.; Brucella spp.; Burkholderia pseudomallei; Campylobacter spp.; Clostridium spp.​​​​; Erysipelothrix spp.; Fusobacterium spp.; Haemophilus spp.; Listeria spp.; Propionibacterium spp.; Salmonella spp.; Yersinia spp.; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house methods MICRO-101; MICRO-101; MICRO-106; MICRO-152; MICRO-153; MICRO-107; MICRO-102; MICRO-100; MICRO-101;  MICRO-108; MICRO-109; MICRO-101; MICRO-104; MICRO-105; MICRO-100; MICRO-101 and MICRO-102

Detection and identification of bacteriaFor production animals, production avian species, Smears; Tissues Clostridium chauvoei; Clostridium novyi; Clostridium septicum; Clostridium sordellii Indirect fluorescent antibody

in-house method MICRO-402


Detection and identification of bacteriaFor production animals Bacterial isolates; Faeces; Tissues Mycobacterium paratuberculosis (Johne's disease) Cultural; Microscopic examination; PCR - Quantitative (qPCR); Staining

in-house methods MICRO-500 (liquid culture); MICRO-502 (solid medium); MICRO-601 (qPCR) and MICRO-501 (staining); ANZSDP - Eamens et al. Paratuberculosis (Johne's Disease) Australian and New Zealand Standard Diagnostic Procedure, July 2015

Faeces Mycobacterium paratuberculosis (Johne's disease) PCR - High throughput direct

in-house method MICRO-600; ANZSDP - Eamens et al. Paratuberculosis (Johne's Disease) Australian and New Zealand Standard Diagnostic Procedure, July 2015

Fluids; Serum; Tissues Clostridium botulinum toxin Enzyme linked immunosorbent assay (ELISA)

in-house methods MICRO-400

Smears; Whole blood Bacillus anthracis Microscopic examination

in-house method MICRO-151

Detection and identification of fungi and moulds Fungal isolates Yeasts MALDI-TOF in-house method MICRO-004 using Bruker MBT Filamentous Library version 4 and in-house method MICRO-007 using custom in-house library
Detection and identification of fungi and mouldsFor bees/apiculture Brood comb Ascosphaera apis Cultural; Microscopic examination

in-house method MICRO-703

Detection and identification of fungi and mouldsFor production animals, production avian species, wildlife, equine species, companion animals, laboratory animals, zoo animals Fluids; Hair; Swabs; Tissues Yeasts Biochemical; Commercial identification kit; Cultural; Microscopic examination

in-house method MICRO-305

Dermatophytes; Identification of unknown fungi; Oomycetes; Zygomycetes Cultural; Microscopic examination

in-house methods MICRO-302; MICRO-301; MICRO-303 and MICRO-306

Detection and identification of other microorganismsFor production animals, production avian species, wildlife, equine species, companion animals, laboratory animals, zoo animals Fluids; Swabs; Tissues Mycoplasma spp. Cultural; Microscopic examination

in-house method MICRO-111

Detection and identification of parasites and arthropodsFor bees/apiculture Whole dead bees Nosema spp. Microscopic examination; Morphological identification

in-house method MICRO-702

Detection and identification of parasites and arthropodsFor production animals Mucus; Preputial smegma; Swabs; Vaginal secretions Tritrichomonas spp. Microscopic examination; Morphological identification

in-house method MICRO-112

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