Biosecurity Queensland Veterinary Laboratories - Department of Agriculture and Fisheries

Site

Biosecurity Sciences Laboratory

Accreditation No.

13389

Site No.

13382

Date of Accreditation

11 Jan 2000

Address

Specimen Receipt (Loading Dock 12), Health and Food Sciences Precinct, 39 Kessels Road
Coopers Plains, QLD 4108
Australia

business.qld.gov.au/industry/agriculture/land-management/health-pests-weeds-diseases/sample-testing

Contact

Dr Louise Jackson P: +61 (07) 37088761

Availability

Services available to external clients

Supervision

Veterinarian Supervised Laboratory

Scope of Accreditation

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Examination of biopsy material Tissues Prion disease lesions Histopathology; Microscopic examination

Australian and New Zealand Standard Diagnostic Procedure, Transmissible Spongiform Encephalopathies, August 2010; in-house method PATH-002.

Histological examination Histopathology; Macroscopic examination; Microscopic examination; Special stains

in-house methods HIS-001 (tissue processing); PATH-002 (histological interpretation); HIS-008 (Congo Red); HIS-009 (Feulgen Reaction); HIS-010 (Giemsa ARI); HIS-047 (DIFF Quick); HIS-013 (Gram); HIS-014 (Grocott's Methamine Silver); HIS-020 (Haematoxylin and eosin-phloxine); HIS-015 (Hall's method-Fouchet's technique); HIS-017 (Luxol Fast Blue); HIS-018 (Mallory Phosphotungstic Acid Haematoxylin); HIS-019 (Masson Trichrome); HIS-023a and HIS-023b (Periodic Acid Schiff's Technique); HIS-024 (Perl's Prussion Blue);  HIS-025 (Phloxine Tartrazine); HIS-026 (Schmorl's Reaction); HIS-029 (Toluidine Blue); HIS-030 (Von-Kossa Technique); HIS-031 (Ziehl Neelsen); HIS-035 (Wolbach's Giemsa); HIS-035 (Warthin-Starry); HIS-050 (Wade-Fite).



Necropsy servicesFor production animals, production avian species, wildlife, aquatic animals, equine species and avian species Body; Tissues Gross pathology and collection of sub-samples Anatomical pathology; Microscopic examination

in-house method PATH-001

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Detection and identification of bacteriaFor avian species Swabs Avibacterium paragallinarum; Pasteurella multocida PCR - Quantitative (qPCR)

in-house methods MOL-028 and MOL-027

Detection and identification of bacteriaFor production animals Tissues; Urine Leptospira spp. PCR - Quantitative (qPCR)

in-house method MOL-042

Detection and identification of parasites and arthropodsFor production animals Preputial smegma; Swabs; Vaginal secretions Tritrichomonas foetus PCR - Quantitative (qPCR)

in-house method MOL-043

Detection and identification of virusesFor aquatic animals (prawns, crabs) Tissues White spot syndrome virus (WSSV) PCR - Quantitative (qPCR) - Reverse transcription in-house method MOL-052
Detection and identification of virusesFor avian species Swabs; Tissues Newcastle disease virus (NDV) PCR - Quantitative (qPCR)

in-house methods MOL-022 and MOL-024

Detection and identification of virusesFor equine species and avian species Swabs; Tissues Influenza virus PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-023 (Influenza Type A)

Detection and identification of virusesFor equine species Culture materials; Swabs; Tissues Equine herpes virus PCR - Quantitative (qPCR) - Multiplex

in-house method MOL-032 (EHV Type 1 & 4 only)

Swabs; Urine; Whole blood Hendra virus PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-033

Detection and identification of virusesFor production animals Buffy coat Bovine immunodeficiency virus; Bovine leukaemia virus; Bovine spumavirus PCR - Quantitative (qPCR); PCR - Quantitative (qPCR) - Multiplex

in-house methods MOL-039; MOL-014

Buffy coat; Culture materials; Swabs; Tissues Bovine viral diarrhoea virus (BVDV) PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-021

Culture materials; Semen; Swabs; Tissues Bovine herpes virus type 5; Bovine herpes virus type 1 PCR - Quantitative (qPCR)

in-house method MOL-036

Lymph nodes; Spleen; Tissues; Whole blood Bluetongue virus (BTV) Cultural; PCR - Quantitative (qPCR) - Reverse transcription

in-house methods VIR-002 and MOL-050 (culture for serotyping and confirmation)

Tissues; Whole blood Bovine ephemeral fever virus PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-029

Detection and identification of virusesFor production avian species, avian species and wildlife Culture materials; Swabs; Tissues Infectious laryngotracheitis virus PCR - Quantitative (qPCR)

in-house method MOL-026

Detection and identification of virusesFor wildlife (bats) Brain tissue; Tissues Australian bat lyssavirus (ABLV) PCR - Quantitative (qPCR) - Reverse transcription

in-house method MOL-038

ISO/IEC 17025 (2017)

Healthcare, Pharmaceutical and Media Products

Service Product Determinant Technique Procedure
Testing for sterility and freedom from contamination of biological materials intended for veterinary use - Innocuity Raw materials of animal origin - Vaccines Bovine herpes virus; Bovine viral diarrhoea virus (BVDV) PCR - Quantitative (qPCR) in-house methods MOL-021 (BVD) and MOL-020 (BoHV-1) 

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Analysis of non-clinical samples for restricted animal material Stockfeeds Blood meal; Fish meal; Meat and bone meal; Meat meal; Poultry meal Microscopic examination

in-house method HIS-039; Australian Ruminant Feed Ban National Guidelines 2013 - 14

Detection and identification of parasites and arthropodsFor production animals, production avian species, avian species, wildlife, equine species, companion animals, laboratory animals and zoo animals Faeces; Gastrointestinal tract specimens; Intestinal contents; Tissues Coccidia; Strongyles; Helminths Microscopic examination; Morphological identification

in-house methods PAR-001; PAR-004

Fresh or preserved parasites Culicoides spp.; Acarina; External bee mites; Identification of unknown parasites; Insects; Screw worm fly; Tracheal bee mites Microscopic examination

in-house methods PAR-031; PAR-003; PAR-027; PAR-002; PAR-032; PAR;027

Arthropods Resistance assays

in-house methods PAR-016 (Larval Packet Test) and PAR-029 (Adult Immersion Test)

Muscle Trichinella spp. Microscopic examination

in-house method PAR-013

Detection and identification of parasites and arthropodsFor production animals Faeces Liver fluke; Stomach fluke Faecal floatation; Sedimentation

In-house method PAR-010 (Breza and Corbe)

Ascaris spp. (roundworm); Capillaria spp.; Coccidia oocyst count; Nematodirus egg count; Strongyles; Strongylid egg count; Strongyloides egg count; Trichirus (whipworm); Cestoda (Cestoidea) Microscopic examination

in-house method PAR-008 (McMaster)

Lungworm larvae Baermann technique

in-house method PAR-009 and ASDT

Chabertia larvae; Cooperia larvae; Haemonchus larvae; Hyostrongylus larvae; Oesophagostomum larvae; Ostertagia larvae; Strongylus larvae; Trichostrongylus larvae; Triodontophorus larvae; Nematodes Cultural; Microscopic examination; Staining

In-house method PAR-011 (larval differentiation)

Faeces; Gastrointestinal tract specimens; Intestinal contents Coccidia; Cryptosporidium spp.; Helminths Concentration; Faecal floatation

in-house methods PAR-01(Anderson method) and PAR-004

Gastrointestinal tract specimens Identification of unknown parasites Microscopic examination

In-house method PAR-012 (total parasite count)

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Detection and identification of virusesFor aquatic animals Plasma; Serum; Swabs; Tissues; Whole blood Aquatic virus; Identification of unknown viruses Cell culture

in-house method VIR-013 and OIE aquatic manual 2009

Detection and identification of virusesFor avian species and production avian species Culture materials Newcastle disease virus (NDV) Haemagglutination inhibition

in-house method SER-031, ASDT

Detection and identification of virusesFor production animals and avian species Buffy coat; Plasma; Semen; Serum; Swabs; Tissues; Whole blood Identification of unknown viruses Cell culture

in-house method VIR-002 (ASDT and/or OIE)

Detection and identification of virusesFor production animals and wildlife (bats) Smears; Tissue imprint; Tissues Australian bat lyssavirus (ABLV) Indirect fluorescent antibody

in-house method SER-035 using commercial monoclonal Ab

Detection and identification of virusesFor production animals, production avian species, avian species, wildlife, equine specieS Plasma; Serum; Swabs; Tissues; Whole blood Identification of unknown viruses Embryonated egg culture

in-house method VIR-001 - allantoic and CAM innoculation

Detection and identification of virusesFor production animals Lymph nodes; Spleen; Tissues; Whole blood Bluetongue virus (BTV) Cultural; PCR - Quantitative (qPCR) - Reverse transcription

in-house methods VIR-002 and MOL-050 (culture for serotyping and confirmation)

Plasma; Serum; Swabs; Tissues; Whole blood Arbovirus Cell culture

in-house method VIR-012 (ASDT 1993)

Immunohistochemistry investigationFor aquatic animals Tissues Nervous necrosis virus (NNV, Betanodovirus) Paraffin section

in-house method SER-060

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor equine species Plasma; Serum Hendra virus soluble G Enzyme linked immunosorbent assay (ELISA) in-house methods SER-069 and SER-024
Equine infectious anaemia virus Agar gel immunodiffusion (AGID) in-house method SER-004, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT) Equine Infectious Anaemia. 1993.
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine and caprine) Plasma; Serum Mycobacterium paratuberculosis (Johne’s disease) - Bovine; Mycobacterium paratuberculosis (Johne’s disease) - Caprine Enzyme linked immunosorbent assay (ELISA) in-house methods SER-025 and SER-025b; manufacturers instructions (Parachek®2 and IDEXX test kits)
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, companion animals, equine species Plasma; Serum Burkholderia pseudomallei Complement fixation test (CFT) in-house method SER-014 
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, production avian species, wildlife, equine species and avian species Plasma; Serum Burkholderia pseudomallei; Newcastle disease virus (NDV) Haemagglutination inhibition

in-house methods SER-033 and SER-031

Serum Leptospira hardjo antibody; Leptospira pomona antibody; Leptospira trassovi antibody Microscopic agglutination

in-house method SER-039 (ASDT)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, wildlife and companion animals (canine) Plasma; Serum Brucella abortus antibody; Brucella suis antibody Rose Bengal plate

in-house method SER-040 (ASDT)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals Plasma; Serum Pestivirus Enzyme linked immunosorbent assay (ELISA) in-house method SER-026 (Pestivirus antigen capture), manufacturers instructions (IDEXX kit)
Mycobacterium paratuberculosis (Johne’s disease) - Caprine Agar gel immunodiffusion (AGID)

in-house method

Brucella ovis Complement fixation test (CFT) in-house method SER-009, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Ovine Brucellosis: Bacteriology and Serology, 1993. 
Enzootic bovine leukosis virus Agar gel immunodiffusion (AGID) in-house method SER-002, Australia and New Zealand Standard Diagnostic Procedures (ANZSDP), Enzootic Bovine Leukosis, 2005.
Campylobacter spp. Enzyme linked immunosorbent assay (ELISA) in-house method SER-073
Epizootic haemorrhagic disease virus Agar gel immunodiffusion (AGID) in-house method SER-003, Australia and New Zealand Standard Diagnostic Procedures (ANZSDP), Epizootic Haemorrhagic Disease. 2014.
Enzootic bovine leukosis virus Enzyme linked immunosorbent assay (ELISA) in-house method SER-022, manufacturers instructions (Svanova Kit)
Caprine retrovirus Enzyme linked immunosorbent assay (ELISA) in-house method SER-021, manufacturers instructions (IDEXX kit)
Bovine viral diarrhoea virus (BVDV); Pestivirus; Mucosal disease Agar gel immunodiffusion (AGID) in-house method SER-007, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Bovine Pestivirus Infections Virology and Serology, 1993.
Aino virus Serum neutralisation

in-house methods SER-052, Australian and New Zealand Standard Diagnostic Procedures (ANZSDP), Aino Virus, 2001. .

Brucella abortus; Brucella suis antibody Complement fixation test (CFT) in-house method SER-008, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Bovine Brucellosis: Serology, 1993.
Bluetongue virus (BTV) Enzyme linked immunosorbent assay (ELISA)

in-house methods SER-072 and SER-072a, manufacturers instructions (IDEXX kit)








Actinobacillus seminis antibody Complement fixation test (CFT)

in-house method SER-011

Neospora caninum antibody Enzyme linked immunosorbent assay (ELISA) in-house method SER-068, manufacturers instructions (IDEXX kit)
Bluetongue virus (BTV) Agar gel immunodiffusion (AGID) in-house method SER-001, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Bluetongue Virology and Serology, 1993, and manufacturers instructions - VMRD.
Serum Bovine viral diarrhoea virus (BVDV); Pestivirus; Mucosal disease Serum neutralisation in-house method SER-049, Australia and New Zealand Standard Diagnostic Procedure (ANZSDP), Ruminent Pestivirus Infections, 2006.
Bovine ephemeral fever virus Serum neutralisation in-house method SER-050
Akabane virus Serum neutralisation in-house method SER-051, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Akabane Disease: Histopathology, Virology and Serology, 1993.
Bovine rhinotracheitis virus Serum neutralisation in-house method SER-048, Australian Standard Diagnostic Techniques for Animal Diseases (ASDT), Infectious Bovine Rhinotracheitis, 1993.
Encephalomyelitis virus Serum neutralisation in-house method SER-053

ISO/IEC 17025 (2017)

Healthcare, Pharmaceutical and Media Products

Service Product Determinant Technique Procedure
Testing for sterility and freedom from contamination of biological materials intended for veterinary use - Innocuity Raw materials of animal origin - Vaccines Bovine herpes virus; Infectious bovine rhinotracheitis virus Virus titration - Quantitative in-house method VIR-008

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Analysis of non-clinical samples for microorganisms Animal feeds; Waters Clostridium botulinum toxin Enzyme linked immunosorbent assay (ELISA)

in-house method MICRO-400

Waters Blue green algae (Cyanobacteria) Microscopic examination

in-house method MICRO-404

Antibiotic sensitivity (susceptibility) testing Bacterial isolates Zone of growth inhibition Disc diffusion

in-house method MICRO-003; Clinical and Laboratory Standards Institute (CLSI) method

Detection and identification of bacteria Bacterial isolates Identification of unknown bacteria MALDI-TOF in-house method MICRO-004 using Bruker Library version V8.0.0.0
Detection and identification of bacteriaFor aquatic animals Fluids; Swabs; Tissues Actinobaculum suis (Eubacterium suis); Alcaligenes spp.; Alteromonas piscicida; Alteromonas rubra; Alteromonas spp.; Bacillus mycoides; Bacillus spp.; Bacillus thuringiensis; Carnobacterium piscicola (Carnobacterium maltaromaticum, Lactobacillus piscicola, Lactobacillus maltaromaticum); Carnobacterium spp.; Corynebacterium aquaticum; Corynebacterium spp.; Edwardsiella ictaluri; Edwardsiella spp.; Edwardsiella tarda; Enterococcus faecalis; Enterococcus hirae; Enterococcus spp.; Flavobacteriacae spp.; Flavobacterium spp.; Flexibacter spp.; Lactococcus garvieae; Lactococcus spp.; Mycobacterium chelonae; Mycobacterium marinum; Mycobacterium spp.; Nocardia spp.; Plesiomonas spp.; Pseudomonas anguilliseptica; Pseudomonas fluorescens; Pseudomonas putida; Pseudomonas spp.; Renibacterium salmoninarum; Streptococcus agalactiae (Streptococcus group B); Streptococcus iniae; Streptococcus spp.; Vibrio spp.; Vibrionaceae; Yersinia ruckeri; Yersinia spp.; Identification of unknown bacteria Biochemical; Cultural; Microscopic examination; Staining in-house methods MICRO-200, MICRO-201, MICRO-202,  MICRO-203, MICRO-205 and MEDIA-002.

Detection and identification of bacteriaFor bees/apiculture Brood comb Melissococcus plutonius; Paenibacillus alvei; Paenibacillus larvae Microscopic examination; Staining

in-house methods MICRO-700 and MICRO-701

Honey Paenibacillus larvae Cultural; Microscopic examination

in-house methods MICRO-700 and MICRO-701

Detection and identification of bacteriaFor production animals, production avian species, avian species, wildlife, aquatic animals, equine species, companion animals, laboratory animals, zoo animals and bees/apiculture Scrapings; Skin; Wounds Dermatophilus spp. Biochemical; Cultural; Microscopic examination; Staining

in-house method MICRO-300

Detection and identification of bacteriaFor production animals, production avian species, wildlife, equine species, companion animals, laboratory animals, zoo animals and bees/apiculture Faeces; Fluids; Swabs; Tissues Actinomyces spp.; Bacteroides spp.; Brachyspira spp.; Brucella spp.; Burkholderia pseudomallei; Campylobacter spp.; Clostridium spp.​​​​; Erysipelothrix spp.; Fusobacterium spp.; Haemophilus spp.; Listeria spp.; Propionibacterium spp.; Salmonella spp.; Yersinia spp.; Identification of unknown bacteria Biochemical; Commercial identification kits; Cultural; Microscopic examination; Staining

in-house methods MICRO-101; MICRO-101; MICRO-106; MICRO-152; MICRO-153; MICRO-107; MICRO-102; MICRO-100; MICRO-101;  MICRO-108; MICRO-109; MICRO-101; MICRO-104; MICRO-105; MICRO-100; MICRO-101 and MICRO-102

Detection and identification of bacteriaFor production animals, production avian species, Smears; Tissues Clostridium chauvoei; Clostridium novyi; Clostridium septicum; Clostridium sordellii Indirect fluorescent antibody

in-house method MICRO-402


Detection and identification of bacteriaFor production animals Bacterial isolates; Faeces; Tissues Mycobacterium paratuberculosis (Johne's disease) Cultural; Microscopic examination; PCR - Quantitative (qPCR); Staining

in-house methods MICRO-500 (liquid culture); MICRO-502 (solid medium); MICRO-601 (qPCR) and MICRO-501 (staining)

Faeces Mycobacterium paratuberculosis (Johne's disease) PCR - High throughput direct

in-house method MICRO-600

Fluids; Serum; Tissues Clostridium botulinum toxin; Clostridium perfringens epsilon toxin Enzyme linked immunosorbent assay (ELISA)

in-house methods MICRO-400 and MICRO-401

Smears; Whole blood Bacillus anthracis Microscopic examination

in-house method MICRO-151

Detection and identification of fungi and moulds Fungal isolates Yeasts MALDI-TOF in-house method MICRO-004 using Bruker Library version V8.0.0.0
Detection and identification of fungi and mouldsFor bees/apiculture Brood comb Ascosphaera apis Cultural; Microscopic examination

in-house method MICRO-703

Detection and identification of fungi and mouldsFor production animals, production avian species, wildlife, equine species, companion animals, laboratory animals, zoo animals Fluids; Hair; Swabs; Tissues Yeasts Biochemical; Commercial identification kits; Cultural; Microscopic examination

in-house method MICRO-305

Dermatophytes; Identification of unknown fungi; Oomycetes; Zygomycetes Cultural; Microscopic examination

in-house methods MICRO-302; MICRO-301; MICRO-303 and MICRO-306

Detection and identification of other microorganismsFor production animals, production avian species, wildlife, equine species, companion animals, laboratory animals, zoo animals Fluids; Swabs; Tissues Mycoplasma spp. Cultural; Microscopic examination

in-house method MICRO-111

Detection and identification of parasites and arthropodsFor bees/apiculture Whole dead bees Nosema spp. Microscopic examination; Morphological identification

in-house method MICRO-702

Detection and identification of parasites and arthropodsFor production animals Mucus; Preputial smegma; Swabs; Vaginal secretions Tritrichomonas spp. Microscopic examination; Morphological identification

in-house method MICRO-112

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