DPIRD Diagnostics and Laboratory Services, Animal Pathology - DDLS - AP - Accredited Organisation (Site No. 13717)

DPIRD Diagnostics and Laboratory Services, Animal Pathology – DDLS – AP

ISO/IEC 17025 (2017)

Food and Beverage

Service	Product	Determinant	Technique	Procedure
Analysis of biotoxin residues and contaminants	Shellfish	13-Desmethyl spirolide C (SPX1); Azaspiracid (AZA); Azaspiracid 1 (AZA1); Azaspiracid 2 (AZA2); Azaspiracid 3 (AZA3); Decarbamoylgonyautoxins 2 and 3 (dcGTX2,3); Decarbamoylneosaxitoxin (dcNEO); Decarbamoylsaxitoxin (dcSTX); Dinophysistoxin 1 (DTX1); Dinophysistoxin 2 (DTX2); Domoic acid (DA); Gonyautoxin 1 and 4 (GTX1,4); Gonyautoxin 2 and 3 (GTX2,3); Gonyautoxin 5 (GTX5, B-1); Gymnodimine (GYM); N-Sulfocarbamoyl-gonyautoxin 1 and 2 (C1,2); Neosaxitoxin (NEO); Okadaic acid (OA); Pectenotoxin-2 (PTX2); Pinnatoxin G (PnTX); Saxitoxin (STX); Tetrodotoxin (TTZ); Yessotoxin (YTX); homo-Yessotoxin (h-YTX)	LC-MS-MS	in-house method BTM-01, 02

DDLS – Anatomical Pathology

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Examination of biopsy material	Tissues	Prion disease lesions	Microscopic examination	The Australian and New Zealand standard diagnostic procedure (ANZSDP) for transmissible spongiform encephalopathies (TSEs), August 2010.
		Histological examination	Histopathology; Immunohistochemistry; Macroscopic examination; Microscopic examination; Special stains	in-house methods PTP-04, PTP-05, PTW-01, PTW-04 (gross exam), PTP-08, PTP-09 (microscopic exam), FHP-15
Immunohistochemistry investigation	Tissues	Bovine viral diarrhoea virus (BVDV); Chlamydia spp.; Porcine circovirus type 2; Distemper	Frozen section; Paraffin section	in-house methods IHC-1, IHC-3 and IHC-2
Necropsy services	Body; Tissues	Gross pathology and collection of sub-samples	Anatomical pathology; Microscopic examination	in-house methods PTP-4, PTP-5, PTW-01, PTW-04, FHP-11, FHP-12 and FHP-13

DDLS – Electron Microscopy

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Detection and identification of virusesFor companion animals, production animals, production avian species, laboratory animals, zoo animals, aquatic animals, wildlife, equine species and avian species	Tissues	Identification of unknown viruses	Transmission electron microscopy (TEM)	in-house methods EMM-1, EMM-2, EMM-5, EMM-6, EMM-7, EMM-8, EMM-9, EMM-10, EMM-11, EMM-12, EMM-13, EMM-14, EMM-15 and EMM-16 Fixatives include: glutaraldehyde, osmium tetraoxide, Stains include: tol blue, uranyl acetate, lead citrate, negative staining,

Service

Product

Determinant

Technique

Procedure

Detection and identification of virusesFor companion animals, production animals, production avian species, laboratory animals, zoo animals, aquatic animals, wildlife, equine species and avian species

Tissues

Identification of unknown viruses

Transmission electron microscopy (TEM)

in-house methods EMM-1, EMM-2, EMM-5, EMM-6, EMM-7, EMM-8, EMM-9, EMM-10, EMM-11, EMM-12, EMM-13, EMM-14, EMM-15 and EMM-16

Fixatives include: glutaraldehyde, osmium tetraoxide,

Stains include: tol blue, uranyl acetate, lead citrate, negative staining,

DDLS – Molecular Biology

ISO/IEC 17025 (2017)

Agribusiness

Service	Product	Determinant	Technique	Procedure
Analysis for insect infestation	Agricultural materials; Insect skin casts; Insect specimens; Larvae; Pupae	Khapra beetle	PCR - Quantitative (qPCR); Polymerase chain reaction (PCR)	in-house method MBM-026
Plant health diagnostics - Viruses, virus-like organisms and viroids	Plant material	Pospiviroid (potato spindle tuber viroid [PSTVd])	Molecular	in-house method PMM-1

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Detection and identification of bacteriaFor production animals (bovine) and avian species	Faeces; Swabs; Tissues; Whole blood	Clostridium botulinum type C toxin; Clostridium botulinum type D toxin	PCR - Quantitative (qPCR); Polymerase chain reaction (PCR)	in-house method MBM-027
Detection and identification of bacteriaFor production animals (ruminants, bovine, ovine, caprine, camelidae, cervidae)	Faeces	Mycobacterium paratuberculosis (Johne's disease)	PCR - Quantitative (qPCR); Polymerase chain reaction (PCR)	in-house method MBM-025
Detection and identification of bacteriaFor production animals	Bacterial isolates; Faeces; Swabs; Tissues	Mycobacterium paratuberculosis (Johne's disease); Mycobacterium paratuberculosis (Johne’s disease) type 1; Mycobacterium paratuberculosis (Johne’s disease) type 2	PCR - Quantitative (qPCR); PCR - Quantitative (qPCR) - Multiplex; Polymerase chain reaction (PCR)	in-house methods MYM-TB6 and MYM-TB3
		Mycobacterium bovis	PCR - Quantitative (qPCR); PCR - Quantitative (qPCR) - Multiplex; Polymerase chain reaction (PCR)	in-house methods MYM-TB5, MYM-TB10 and MYW-15
Detection and identification of parasites and arthropods	Fresh or preserved parasites	Screw worm fly	PCR - Quantitative (qPCR); Polymerase chain reaction (PCR)	in-house method MBM-028
Detection and identification of viruses	Whole blood	Ovine herpes virus 2 (malignant catarrhal fever)	Polymerase chain reaction (PCR)	in-house method VIM-11
Detection and identification of virusesFir production animals (bovine), equine species and avian species	Faeces; Swabs; Tissues; Whole blood	Avian influenza H5 genome; Avian influenza H7; Bluetongue virus (BTV); Foot and mouth disease virus (FMD); Hendra virus; Influenza A virus; Newcastle disease virus (NDV)	PCR - Quantitative (qPCR) - Reverse transcription; Polymerase chain reaction (PCR)	in-house methods MBM-001 (FluA), MBM-002 (NDV), MBM-003 (Hendra), MBM-004 (FMD), MBM-005 (BTV)

ISO/IEC 17025 (2017)

Environment

Service	Product	Determinant	Technique	Procedure
Detection, characterisation and/or quantification of nucleic acids	DNA	Annelida; Arthropoda; Chlorophyta; Chordata - Ascidian; Chordata - Fish; Cnidaria; Ctenophora; Echinodermata; Mollusca; Ochrophyta; Platyhelminthes; Porifera; Rhodophyta	Barcode of life database (BOLD); DNA barcoding; Extraction; International nucleotide sequence database collaborative (INSDC); Polymerase chain reaction (PCR)	in-house method BBM-0

DDLS – Parasitology

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Detection and identification of parasites and arthropodsFor bees/apiculture	Fresh or preserved parasites	Tropilaelaps mite; Varroa mite; Braula fly	Microscopic examination	in-house method PAM-37
Detection and identification of parasites and arthropodsFor production animals (Bovine, camelids, caprine, ovine, porcine), production avian species, zoo animals, equine species	Muscle; Scrapings; Tissues	Identification of unknown parasites	Microscopic agglutination; Microscopic examination	in-house method PAM-30
Detection and identification of parasites and arthropodsFor production animals (Bovine, camelids, caprine, ovine, porcine), production avian species, zoo animals, equine species	Faeces	Coccidia oocyst count; Moniezia egg count; Nematodirus egg count; Strongylid egg count; Strongyloides egg count; Trichuris egg count	Microscopic examination	in-house method PAM-18
		Cryptosporidium oocyst; Giardia cyst; Giardia oocyst; Giardia trophozoites	Direct immunofluorescence; Microscopic examination	in-house method PAM-26
	Faeces; Intestinal contents; Scrapings	Identification of unknown parasites	Microscopic examination; Staining	in-house methods PAM-33 (formol ether concentration), PAM-34 (giemsa and Diff-Quik® stains)
Detection and identification of parasites and arthropodsFor production animals (Bovine, camelids, caprine, ovine, porcine), zoo animals, equine species	Faeces	Cryptosporidium spp.	Immunochromatography	in-house method PAM-36
	Faeces; Fluids; Urine	Chabertia larvae; Cooperia larvae; Haemonchus larvae; Hyostrongylus larvae; Oesophagostomum larvae; Ostertagia larvae; Strongylus larvae; Triodontophorus larvae	Cultural; Microscopic examination; Staining	in-house method PAM-25 (larval differentiation)
Detection and identification of parasites and arthropodsFor production animals (Bovine, caprine, ovine), zoo animals	Faeces	Elaphostrongylus cervi larvae; Lungworm larvae	Baermann technique	Australian standard diagnostic technique (ASDT), in-house method PAM-29
Detection and identification of parasites and arthropodsFor production animals (Bovine, camelids, caprine, ovine, porcine), production avian species, zoo animals	Faeces; Fluids; Urine	Identification of unknown helminth oocysts, cysts and eggs; Identification of unknown protozoa oocysts, cysts and eggs	Faecal floatation; Microscopic examination	in-house methods PAM-19 and PAM-32 (concentration flotation, centrifugal flotation)
Detection and identification of parasites and arthropodsFor production animals (Bovine, camelids, caprine, ovine, porcine), zoo animals, equine species	Faeces	Liver fluke; Stomach fluke	Sedimentation	Department of Food and Agriculture approved fluke egg sedimentation test (FEST) procedure, in-house method PAM-17
Detection and identification of parasites and arthropodsFor production animals (Bovine, caprine, ovine, porcine), zoo animals	Faeces	Haemonchus contortus eggs	Direct immunofluorescence; Microscopic examination; Staining	in-house method PAM-28 (Lectin stain)

DDLS – Serology

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Analysis of non-clinical samples for microorganisms	Fines; Grains; Grass; Hay; Seeds	Rathayibacter toxicus	Enzyme linked immunosorbent assay (ELISA)	in-house methods IMM-1 (standard ELISA), IMM-2 (short ELISA) and IMM-12 (quick ELISA)
Detection and identification of bacteriaFor production animals	Serum	Corynebacterium pseudotuberculosis	Enzyme linked immunosorbent assay (ELISA)	in-house methods IMM-6 (toxin antibody) and IMM-7 (cell wall)
Detection and identification of bacteriacompanion animals, production animals, production avian species, laboratory animals, zoo animals, wildlife, aquatic animals, equine species, avian species and apiculture	Smears; Tissues	Chlamydia spp.	Indirect fluorescent antibody	in-house method VIM 10.1
Detection and identification of parasites and arthropodsFor production animals	Faeces	Liver fluke	Enzyme linked immunosorbent assay (ELISA)	Manufacturers instructions - Bio-X Diagnostics kit
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor companion animals, production animals, production avian species, laboratory animals, zoo animals, wildlife, equine species and avian species	Serum	Clostridium perfringens type E	Enzyme linked immunosorbent assay (ELISA)	in-house method IMM-10 Manufacturers Instructions - BioX Diaignostics
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, equine species	Plasma; Serum	Epizootic haemorrhagic disease virus; Equine infectious anaemia virus	Agar gel immunodiffusion (AGID)	in-house method VIM-2
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, production avian species, equine species and avian species	Plasma; Serum	Akabane virus; Bluetongue virus (BTV); Bovine viral diarrhoea virus (BVDV); Caprine arthritis encephalitis virus; Douglas virus; Hendra virus; Influenza A virus; Mycoplasma ovis (Eperythrozoon ovis) antibody; Peaton virus; Sabo virus; Sathuperi virus; Schmallenburg virus; Shamoda virus; Simbu virus; Tinaroo virus group-reactive; Infectious bovine rhinotracheitis (IBR)Bovine viral diarrhoea virus (BVDV)- type 1	Enzyme linked immunosorbent assay (ELISA)	in-house methods VIM-2.27 (AKA - IDVet kit), VIM-2.26 (BTV - IDEXX and IDVet kits), VIM-2.20 (BVDV - IDEXX kit), VIM-2.11B (CAE - VMRD kit), VIM - (Simbu - IDEXX Schmallenberg kit), VIM-2.29 (Hendra - in-house method), VIM-2.17, IMM-8 (m.ovis - in-house method), VIM-2.25 (IBR - IDVet kit)
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals	Bulk milk; Milk; Plasma; Serum	Bovine leukaemia virus	Enzyme linked immunosorbent assay (ELISA)	in-house methods VIM2.15 and VIM2.13B Manufacturers instructions - Synbiotics kits
	Serum	Brucella abortus antibody; Brucella melitensis antibody; Brucella suis antibody	Serum agglutination	Australian standard diagnostic technique (ASDT) and in-house method SEM-3
		Brucella abortus; Brucella melitensis; Brucella ovis; Coxiella burnetii (Q-fever) antibody; Johne's disease	Complement fixation test (CFT)	Australian standard diagnostic technique (ASDT) and in-house method SEM-7
		Brucella abortus antibody; Brucella melitensis antibody; Brucella suis antibody	Rose Bengal plate	Australian standard diagnostic technique (ASDT) and in-house method SEM-4
		Bovine venereal campylobacteriosis; Neospora caninum antibody	Enzyme linked immunosorbent assay (ELISA)	in-house methodd IMM-5 (BVC), SEM-9 (Neospora canimum Ab)
		Johne's disease	Enzyme linked immunosorbent assay (ELISA)	in-house methods SEM-6a Manufacturers instructions: Prionics Paracheck screen kit (Bovine/Ovine/Caprine)
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production avian species and avian species	Plasma; Serum	Egg drop syndrome virus; Newcastle disease virus (NDV)	Haemagglutination inhibition	in-house method VIM-4

DDLS – SSBA

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Analysis of non-clinical samples for microorganisms	Animal feeds	Clostridium botulinum type C toxin; Clostridium botulinum type D toxin	Enzyme linked immunosorbent assay (ELISA)	in-house method SEM-14
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, avian species	Serum; Tissues	Clostridium botulinum type C toxin; Clostridium botulinum type D toxin	Enzyme linked immunosorbent assay (ELISA)	in-house method SEM-14
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals	Serum	Clostridium botulinum type C toxin; Clostridium botulinum type D toxin	Enzyme linked immunosorbent assay (ELISA)	in-house method IMM-4

DDLS – Virology

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Analysis for transmissible animal diseases under the European Union Directives for Animal Health	Equine reproductive material	Equine arteritis virus; Equine infectious anaemia	Virus isolation	Australian and New Zealand standard diagnostic procedure (ANZSDP), World Organisation for Animal Health (OIE) methods
Detection and identification of virusesfor companion animals, production animals, production avian species, laboratory animals, zoo animals, wildlife, equine species and avian species	Serum; Swabs; Tissues	Akabane virus; Bovine ephemeral fever virus; Equine arteritis virus; Equine herpes virus; Infectious bovine rhinotracheitis virus; Parainfluenza virus type 3; Pestivirus; Identification of unknown viruses	Cell culture; Virus isolation	Australian and New Zealand standard diagnostic procedure (ANZSDP), in-house methods VIM-6.1 and VIM-6.2, World Organisation for Animal Health (OIE)
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals, production avian species, equine species and avian species	Plasma; Serum	Aino virus; Akabane virus; Ephemeral fever virus; Equine arteritis virus; Equine herpes virus type 1; Parainfluenza virus type 3; Ross River virus; Infectious bovine rhinotracheitis (IBR)Bovine viral diarrhoea virus (BVDV) type 1	Serum neutralisation	in-house method VIM-3

DDLS Bacteriology

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Analysis of non-clinical samples for microorganisms	Mulch	Escherichia coli serotyping; Escherichia coli; Salmonella spp.; Salmonella serotype	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	AS 5013.3, AS 5013.15, ISO 4831, ISO 7251 in-house method BAM-22
	Waters	Blue green algae (Cyanobacteria)	Cultural; Microscopic examination	in-house method BAM-19
Analysis of non-clinical samples for microorganismsFor avian species	Animal feeds; Chick paper; Waters	Salmonella spp.	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-18
Antibiotic sensitivity (susceptibility) testing	Bacterial isolates; Fungal isolates	Zone of growth inhibition	Disc diffusion	Clinical and Laboratory Standards Institute (CLSI), Australian and New Zealand standard diagnostic procedure (ANZSDP), in-house method BAM-4
Detection and identification of bacteria	Bacterial isolates	Identification of unknown bacteria	MALDI-TOF	work instruction BAW 4.1 (MBT 7854 MSP Library)
Detection and identification of bacteriaFor aquatic animals	Bacterial isolates; Swabs; Tissues	Aerococcus viridans; Aeromonas caviae; Aeromonas hydrophila; Aeromonas salmonicida; Aeromonas sobria; Aeromonas spp.; Aphanomyces astaci; Carnobacterium piscicola (Carnobacterium maltaromaticum, Lactobacillus piscicola, Lactobacillus maltaromaticum); Citrobacter freundii; Clostridium botulinum; Edwardsiella ictaluri; Edwardsiella tarda; Enterococcus seriolicida; Eubacterium tarantellae; Flavobacterium branchiophilum; Mycobacterium fortuitum; Mycobacterium marinum; Nocardia spp.; Photobacterium damselae ss. damselae (Vibrio damselae ss. damselae); Photobacterium damselae ss. piscicida (Pasteurella piscicida); Pseudomonas anguilliseptica; Pseudomonas fluorescens; Staphylococcus delphini; Streptococcus agalactiae (Streptococcus group B); Tenacibaculum maritimum (Flexibacter maritimum); Vagococcus salmoninarum; Vibrio alginolyticus; Vibrio furnissii; Vibrio harveyi; Vibrio jasicida; Vibrio mimicus; Vibrio ordalii; Vibrio owensii; Vibrio parahaemolyticus; Vibrio penaeicida; Vibrio proteolyticus; Vibrio rotiferianus; Vibrio salmonicida; Vibrio splendidus; Vibrio tapetis; Vibrio tasmaniaensis; Vibrio tubiashii; Vibrio vulnificus biotype 2; Yersinia ruckeri; Identification of unknown bacteria	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-9 (Aquatic culture)
Detection and identification of bacteriaFor bees/apiculture	Honey; Larval smear	Ascosphaera apis - Chalk brood 1; Ascosphaera apis - Chalk brood 2; Melissococcus plutonius; Nosema apis; Nosema ceranae; Paenibacillus larvae; Identification of unknown bacteria	Biochemical; Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-5
Detection and identification of bacteriaFor companion animals, production animals, production avian species, laboratory animals, zoo animals, wildlife, aquatic animals, equine and avian species, bees/apiculture	Bacterial isolates	Escherichia coli; Coliforms; Plate count	Cultural	in-house method BAM-7
	Bacterial isolates; Swabs; Tissues	Actinobaculum suis (Eubacterium suis); Actinomyces bovis; Bacteroides fragilis; Bacteroides melaninogenicus; Brachyspira hampsonii; Brachyspira hyodysenteriae; Brachyspira pilosicoli; Clostridium chauvoei; Clostridium novyi; Clostridium perfringens; Clostridium septicum; Clostridium sordellii; Clostridium spiroforme; Clostridium sporogenes; Clostridium spp.; Fusobacterium necrophorum; Fusobacterium nucleatum; Fusobacterium russii; Peptoniphilus indolicus (Peptostreptococcus indolicus); Porphyromonas asaccharolytica; Porphyromonas levii (Bacteroides levii); Prevotella heparinolytica; Prevotella melaninogenica; Identification of unknown bacteria	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-2
	Swabs; Tissues	Mycobacterium bovis; Mycobacterium paratuberculosis (Johne's disease); Mycobacterium spp.	Cultural; Microscopic examination	in-house methods MYM-TB1, MYM-JD1, MYM-JD7, MYM-JD8, BAM-13
Detection and identification of bacteriaFor companion animals, production animals, production avian species, laboratory animals, zoo animals, wildlife, aquatic animals, equine species, avian species	Abortion material; Reproductive tract specimens	Campylobacter fetus ss. fetus; Campylobacter fetus ss. venerealis (Cfv); Identification of unknown bacteria	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-15 (breeding screen)
	Bacterial isolates; Respiratory specimens; Swabs; Tissues	Pasteurella spp.; Streptococcus equi; Identification of unknown bacteria	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-16
	Bacterial isolates; Swabs; Tissues	Burkholderia pseudomallei; Pseudomonas mallei	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-6
		Rhodococcus equi	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-10
Detection and identification of bacteriaFor laboratory animals	Bacterial isolates; Swabs; Tissues	Bordetella bronchiseptica; Corynebacterium kutcheri; Klebsiella pneumoniae; Pasteurella multocida; Pasteurella pneumotropica; Pseudomonas aeruginosa; Salmonella enterica serovar Typhimurium; Staphylococcus aureus; Streptobacillus moniliformis; Streptococcus pneumoniae; Streptococcus pyogenes; Identification of unknown bacteria	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-11 (laboratory animal screening)
Detection and identification of bacteriaFor production animals	Milk	Achromobacter spp.; Acinetobacter spp.; Actinomyces spp.; Aerococcus viridans; Aeromonas spp.; Bacillus cereus; Bacillus spp.; Chryseobacterium spp.; Citrobacter spp.; Corynebacterium spp.; Enterobacter spp.; Enterococcus spp.; Escherichia coli; Ewingella spp.; Flavobacterium spp.; Hafnia alvei; Klebsiella oxytoca; Klebsiella pneumoniae; Kocuria spp.; Lactobacillus spp.; Leuconostoc spp.; Micrococcus spp.; Pantoea spp.; Prototheca spp.; Pseudomonas spp.; Serratia spp.; Staphylococcus aureus; Streptococcus agalactiae (Streptococcus group B); Vagococcus spp.; Identification of unknown bacteria	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-23 (milk hygiene testing)
Detection and identification of bacteriaFor production animals	Bacterial isolates; Swabs; Tissues	Bacillus anthracis	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-3
		Brachyspira hyodysenteriae; Brachyspira innocens; Brachyspira murdochii; Brachyspira pilosicoli; Campylobacter coli; Campylobacter fetus; Campylobacter jejuni; Clostridium perfringens; Escherichia coli; Lawsonia spp.; Salmonella spp.; Yersinia enterocolitica; Yersinia pseudotuberculosis	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-8
	Milk	Enterobacter aerogenes; Escherichia coli; Klebsiella pneumoniae; Peptoniphilus indolicus (Peptostreptococcus indolicus); Staphylococcus aureus; Streptococcus agalactiae (Streptococcus group B); Streptococcus equi; Streptococcus pneumoniae; Streptococcus pyogenes; Streptococcus uberis; Trueperella pyogenes; Identification of bacteria causing mastitis	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-12 (mastitis culture)
Detection and identification of bacteriaFor zoo animals	Bacterial isolates; Faeces; Swabs; Tissues	Campylobacter spp.; Escherichia coli; Salmonella spp.; Shigella spp.; Yersinia spp.; Identification of unknown bacteria	Biochemical; Biochemical - Automated (VITEK); Commercial identification kits; Cultural; Microscopic examination; Staining	in-house method BAM-21 (zoo animal testing)
Detection and identification of fungi and mouldsFor companion animals, production animals, production avian species, laboratory animals, zoo animals, wildlife, aquatic animals, equine and avian species, bees/apiculture	Fungal isolates; Hair; Nails; Scabs; Skin; Swabs; Tissues	Absidia spp.; Aspergillus flavus; Aspergillus fumigatus; Candida albicans; Cryptococcus gattii; Cryptococcus neoformans; Microsporum spp.; Mortierella spp.; Mucor spp.; Rhizomucor; Rhizopus; Trichophyton spp.; Identification of unknown fungi	Biochemical - Automated (VITEK); Cultural; Microscopic examination; Staining	in-house method BAM-14
Detection and identification of other microorganismsFor companion animals, production animals, production avian species, laboratory animals, zoo animals, wildlife, aquatic animals, equine and avian species, bees/apiculture	Swabs; Tissues	Mycoplasma spp.	Cultural; Microscopic examination	in-house method BAM-13
		Mycoplasma spp.	Cultural; Microscopic examination	in-house methods BAM-13

DDLS Biochemistry

ISO/IEC 17025 (2017)

Animal Health

Service	Product	Determinant	Technique	Procedure
Analysis for trace elements	Plasma; Tissues; Whole blood	Copper; Zinc	Atomic absorption spectroscopy (AAS) - Flame; Spectrophotometry	in-house methods NTM-21 and NTM-52
		Selenium	Atomic absorption spectroscopy (AAS) - Flame	in-house method NTM-24
	Tissues	Lead	Atomic absorption spectroscopy (AAS) - Flame	in-house method NTM-26
	Whole blood	Lead	Electrode stripping voltammetry (ESA)	in-house method NTW-62
Analysis of non-clinical samples for residues and contaminants	Animal feeds	Amino acids; Retinol (vitamin A); Tocopherol (vitamin E)	HPLC	in-house methods NTR-17 (amino acids), NTM-31 (vit A & E)
		Fatty acids - Total	Gas liquid chromatography (GLC)	in-house method NTR-16
		Monofluoroacetate	HPLC; Mass spectrometry (MS)	in-house method NTM-TOX-1
		Selenium	Atomic absorption spectroscopy (AAS) - Flame	in-house method NTM-68
	Waters	Conductivity; pH	Direct measuring instrument	Greenberg Arnold,E.and Clesceri Lenore, S.,1992. Standard methods for the examinationof water and waste water. (Conductivity and pH); in-house method NTW-56
Chemical pathology - Investigation and determination of hepatic, cardiac, bone, skeletal muscle and other profiles and metabolic studiesFor companion animals, production animals, production avian species, laboratory animals, zoo animals, aquatic animals, wildlife, equine species and avian species	Plasma; Serum	Methylmalonic acid (MMA)	GC-MS	in-house method NTM-33
	Plasma; Tissues	Acetylcholinesterase (RCAC)	Spectrophotometry	in-house method NTM-TOX-2
	Rumen	pH	Direct measuring instrument	in-house method NTW-56
	Tissues	Fatty acids - Total	Gas liquid chromatography (GLC)	in-house method NTR-16
Chemical pathology - Investigation and determination of hepatic, cardiac, bone, skeletal muscle and other profiles and metabolic studiesfor companion animals, production animals, production avian species, laboratory animals, zoo animals, aquatic animals, wildlife, equine species and avian species	Fluids; Plasma; Serum	Alanine aminotransferase; Albumin; Bilirubin - Direct; Bilirubin - Total; Calcium; Cholesterol; Creatinine; Creatinine kinase; Glutamine dehydrogenase; Glutathione peroxidase; Haptoglobins; Iron; Magnesium; Phosphate; Protein - Total; Urea; β-Hydroxybutyrate; γ-Glutamyltransferase	Autoanalyser (AA)	Olympus AU480
Quantitation of vitaminsFor companion animals, production animals, production avian species, laboratory animals, zoo animals, aquatic animals, wildlife, equine species and avian species	Liver; Plasma	Retinol (vitamin A); Tocopherol (vitamin E)	HPLC - Electrochemical detector	in-house methods NTM-31 (tissue), NTM-32 (plasma)
	Whole blood	Thiamine (vitamin B1)	HPLC - Electrochemical detector	in-house method NTM-50

ISO/IEC 17025 (2017)

Food and Beverage

Service	Product	Determinant	Technique	Procedure
Analysis of biotoxin residues and contaminants	Shellfish	13-Desmethyl spirolide C (SPX1); Azaspiracid (AZA); Azaspiracid 1 (AZA1); Azaspiracid 2 (AZA2); Azaspiracid 3 (AZA3); Decarbamoylgonyautoxins 2 and 3 (dcGTX2,3); Decarbamoylneosaxitoxin (dcNEO); Decarbamoylsaxitoxin (dcSTX); Dinophysistoxin 1 (DTX1); Dinophysistoxin 2 (DTX2); Domoic acid (DA); Gonyautoxin 1 and 4 (GTX1,4); Gonyautoxin 2 and 3 (GTX2,3); Gonyautoxin 5 (GTX5, B-1); Gymnodimine (GYM); N-Sulfocarbamoyl-gonyautoxin 1 and 2 (C1,2); Neosaxitoxin (NEO); Okadaic acid (OA); Pectenotoxin-2 (PTX2); Pinnatoxin G (PnTX); Saxitoxin (STX); Tetrodotoxin (TTZ); Yessotoxin (YTX); homo-Yessotoxin (h-YTX)	LC-MS-MS	in-house method BTM-01, 02

Department of Primary Industries and Regional Development