NSW Department of Primary Industries

Site

Elizabeth Macarthur Agricultural Institute – Veterinary Testing

Accreditation No.

14495

Site No.

14520

Date of Accreditation

18 Feb 2021

Address

Woodbridge Road
Menangle, NSW 2568
Australia

dpi.nsw.gov.au

Contact

Ms Pauline Stamedes P: +61 0418207193

Availability

Services available to external clients

Supervision

Veterinarian Supervised Laboratory

Scope of Accreditation

EMAI – Biotechnology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Detection and identification of bacteriaFor production animals (bovine, ovine) Faeces Mycobacterium paratuberculosis (Johne's disease) PCR - High throughput direct

in-house method DNA-M3-002

Inherited traitsFor production animals (bovine, ovine) and equine species Extracted DNA; Hair; Semen; Tissues; Whole blood A2 milk protein; Bovine leukocyte adhesion deficiency (BLAD); Chediak-Higashi syndrome; Citrullinemia; Coat colour recessive red; Dermatosparaxis; Double muscling - 821del11; Double muscling - C313Y; E18 Pompes disease; E7 Pompes disease; Factor XI deficiency in Holstein Friesians; Factor XII deficiency; Freemartinism; Hereditary spherocytosis in Wagyu; Inherited congenital myoclonus (ICM); Maple syrup urine disease - Hereford; Maple syrup urine disease - Shorthorn; Myophosphorylase deficiency; Protoporphyria; Renal tube dysplasia - Claudin 16 in Wagyu; α-Mannosidosis - Angus; α-Mannosidosis - Galloway; β-Mannosidosis; κ-Casein PCR - Quantitative (qPCR); PCR - Restriction fragment length polymorphism

in-house method DNA-M3-001

EMAI – MaP – Bacteriology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Analysis of non-clinical samples for microorganisms Environmental samples Salmonella spp. Biochemical; Cultural; Microscopic examination; Slide agglutination in-house method PMBAC-123 (Salmonella isolation and identification)
Antibiotic sensitivity (susceptibility) testing Bacterial isolates Zone of growth inhibition Disc diffusion

in-house method WBAC-001, Antibiotic Susceptibility Testing by the CDS Method - A Manual for Medical and Veterinary Laboratories

Detection and identification of bacteria Bacterial isolates Identification of unknown bacteria MALDI-TOF in-house method PMBAC-401; Bruker library version: MBT Compass Revision H
Identification of unknown bacteria Biochemical; Commercial identification kit; Sequencing

in-house methods: 

MMBAC-113 (Plate reading - General guidelines); 

MMBAC-500 (Follow-up Methods and Other Tests)

Detection and identification of bacteriaFor bees/apiculture Brood comb; Honey; Larval smear; Mummified larvae Ascosphaera apis - Chalk brood 1; Ascosphaera apis - Chalk brood 2; American foulbrood (AFB); European foulbrood (EFB) Biochemical; Cultural; Microscopic examination; Staining

in-house methods:

PMBAC-121 (American foulbrood), 

PMBAC-122 (Preparation and Examination of Larval Smears), and:

PMBAC-108 

Detection and identification of bacteriaFor production animals (bovine, porcine, ovine, camelid), companion animals (canine, feline), wildlife, equine species and avian species Fluids; Swabs Escherichia coli​​​​; Staphylococcus spp.; Streptococcus spp.; Trueperella pyogenes; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house methods:

MMBAC-102 (Body Fluids - General approach for peritoneal, pericardial and pleural fluid Microscopy and Culture)

MMBAC-500 (Follow-up Methods and Other Tests)
Detection and identification of bacteriaFor production animals (bovine, ovine, caprine, camelid, porcine) and equine species Fluids; Smears Bacillus anthracis Staining

in-house method PMBAC-141 (Polychrome methylene blue stain)

Detection and identification of bacteriaFor production animals (bovine, porcine, caprine), companion animals (canine, feline) and equine species Milk; Whole blood Identification of bacteria causing mastitis Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house method MMBAC-111 (Milk - culture for mastitis)

Detection and identification of bacteriaFor production animals (bovine, porcine, caprine), companion animals (canine, feline), wildlife (mollusc), aquatic animals, equine species and avian species Liver; Tissues Actinobacillus equuli; Actinobacillus ligneresi; Actinobacillus pleuropneumoniae; Actinobacillus seminis; Actinobacillus spp.; Actinobaculum suis (Eubacterium suis); Actinomyces bovis; Actinomyces spp.; Aeromonas caviae; Aeromonas sobria; Aeromonas spp.; Bacillus anthracis; Bacillus cereus; Bacteroides spp.; Bordetella bronchiseptica; Brucella spp.; Clostridium perfringens; Corynebacterium pseudotuberculosis; Corynebacterium renale; Corynebacterium spp.; Cronobacter sakazakii (Enterobacter sakazakii); Dermatophilus congolensis; Dichelobacter nodosus (footrot); Empedobacter brevis; Enterobacter cloacae; Enterobacter gergoviae; Enterobacter intermedius; Enterobacter spp.; Enterococcus faecalis; Enterococcus faecium; Enterococcus spp.; Erysipelothrix rhusiopathiae; Escherichia coli - Haemolytic; Escherichia coli - Non-haemolytic; Escherichia coli​​​​; Escherichia spp.; Flavobacterium spp.; Fusobacterium necrophorum; Haemophilus parasuis; Histophilus somni (Histophilus ovis); Klebsiella pneumoniae; Klebsiella spp.; Listeria ivanovi; Listeria monocytogenes; Listeria spp.; Mannheimia haemolytica; Moraxella spp.; Morganella morganii; Nocardia asteroides; Nocardia spp.; Pasteurella gallinarum; Pasteurella multocida; Pasteurella spp.; Peptostreptococcus spp.; Proteus spp.; Providencia stuartii; Pseudomonas aeruginosa; Rhodococcus equi; Salmonella spp.; Serratia marcescens; Staphylococcus aureus; Staphylococcus epidermidis; Staphylococcus hyicus; Staphylococcus intermedius; Streptobacillus moniliformis; Streptococcus agalactiae (Streptococcus group B); Streptococcus bovis; Streptococcus canis; Streptococcus dysgalactiae ss. dysgalactiae; Streptococcus dysgalactiae ss. equisimilis; Streptococcus equi ss. equi; Streptococcus equi ss. zooepidemicus; Streptococcus mitis; Streptococcus mutans; Streptococcus pneumoniae; Streptococcus pyogenes; Streptococcus salivarius; Streptococcus sanguinis; Streptococcus spp.; Streptococcus suis serotypes 1/2/9; Streptococcus uberis; Streptococcus - Alpha-haemolytic; Streptococcus - Beta-haemolytic; Streptomyces spp.; Trueperella pyogenes; Vibrio cholerae; Yersinia enterocolitica; Yersinia kristensenii; Yersinia pseudotuberculosis; Yersinia ruckeri; Yersinia spp.; Identification of unknown bacteria Cultural; Microscopic examination; Staining

in-house method MMBAC-110 (The Liver (and other tissues) - General guidelines for tissues microscopy and culture) and PMBAC-407 (Anaerobic Culture)

Detection and identification of bacteriaFor production animals (bovine, porcine, caprine), companion animals (canine, feline), wildlife (snake), equine species and avian species Abortion material; Bacterial isolates; By-products; Chick bodies; Faeces; Tissues; Whole blood Salmonella spp. Biochemical; Cultural; Microscopic examination; Serum agglutination

in-house methods

PMBAC-123 (Salmonella isolation and identification);

MMBAC-500 (Follow-up Methods and Other Tests)
Detection and identification of bacteriaFor production animals (bovine, porcine, caprine), companion animals (canine, feline), wildlife, equine species and avian species Abortion material; Brain tissue; Cerebrospinal fluid (CSF); Pus; Tissues Listeria spp. Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house method MMBAC-109 (Listeria Isolation and identification)

Central nervous system specimens; Cerebrospinal fluid (CSF); Exudate; Fluids; Pus; Tissues Actinomyces spp.; Escherichia coli​​​​; Histophilus haemophilia; Histophilus somni (Histophilus ovis); Listeria monocytogenes; Rhodococcus equi; Staphylococcus aureus; Streptococcus pyogenes; Streptococcus suis; Streptococcus - Beta-haemolytic; Trueperella pyogenes; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house method PMBAC-110 (microbial causes of CNS infections)

Exudate; Pus; Skin; Swabs Actinobacillus ligneresi; Actinomyces bovis; Actinomyces viscosus; Arcanobacteria; Candida albicans; Clostridium perfringens; Corynebacterium pseudotuberculosis; Erysipelothrix rhusiopathiae; Nocardia asteroides; Pasteurella dagmatis; Pasteurella multocida; Proteus spp.; Pseudomonas aeruginosa; Sporothrix schenckii; Staphylococcus aureus; Staphylococcus hyicus; Staphylococcus intermedius; Staphylococcus spp.; Streptococcus pyogenes; Streptococcus spp.; Streptococcus group C; Streptococcus group E; Trueperella pyogenes; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house methods:

MMBAC-116 (The skin and subcutis - Microscopy and culture)

MMBAC-500 (Follow-up Methods and Other Tests)
Exudate; Pus; Swabs; Tissues; Urinary system specimens; Urine Actinobacillus spp.; Actinobaculum suis (Eubacterium suis); Actinomyces spp.; Clostridium perfringens; Corynebacterium renale; Escherichia coli​​​​; Proteus spp.; Staphylococcus aureus; Staphylococcus spp.; Streptococcus pyogenes; Streptococcus spp.; Trueperella pyogenes; Coliforms; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house method PMBAC-128 (The urinary system - Microscopy and culture)

Faeces; Intestinal contents; Swabs Campylobacter spp.; Clostridium perfringens; Salmonella spp.; Yersinia spp.; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house methods:

MMBAC-105 (The gastrointestinal (GIT) tract - Microscopy and culture); 

PMBAC-123 (Salmonella isolation and identification), and; 

MMBAC-122 (Yersinia isolation and identification).

Skin Dermatophilus congolensis Cultural; Microscopic examination; Staining in-house method MMBAC-116 (Skin and Subcutis – Microscopy and Culture)
Smears Identification of unknown bacteria Microscopic examination

in-house method MMBAC-112 (Stereoscopic microscopy,  Brightfield microscopy, Phase microscopy,  Darkfield microscopy,  Fluorescence microscopy)

Detection and identification of bacteriaFor production animals (bovine, porcine, caprine, camelid), companion animals (canine, feline), equine species and avian species Bone; Synovial fluid; Synovial membrane; Tissues Actinobacillus equuli; Actinomyces bovis; Erysipelothrix rhusiopathiae; Escherichia coli​​​​; Histophilus somni (Histophilus ovis); Nocardia spp.; Salmonella spp.; Staphylococcus aureus; Staphylococcus intermedius; Staphylococcus spp.; Streptococcus spp.; Streptococcus group C; Trueperella pyogenes; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house methods MMBAC-108 (Joints and bone - Microscopy and culture) and MMBAC-500 (Follow-up Methods and Other Tests)

Clitoral samples; Exudate; Faeces; Fluids; Foetal specimens; Gastrointestinal tract specimens; Intestinal contents; Mucus; Reproductive tract specimens; Scrapings; Swabs; Tissues; Uterine samples Arcobacter spp.; Campylobacter coli; Campylobacter fetus ss. fetus; Campylobacter fetus ss. venerealis (Cfv); Campylobacter fetus; Campylobacter hyointestinalis; Campylobacter jejuni; Campylobacter spp.; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house method PMBAC-103 (Campylobacter spp. isolation and identification)

Detection and identification of bacteriaFor production animals (bovine, porcine, ovine, camelid), companion animals (canine, feline), wildlife, equine species and avian species Exudate; Fluids; Mucus; Reproductive tract specimens; Scrapings; Semen; Swabs; Tissues; Uterine samples Actinobacillus spp.; Arcobacter spp.; Bacillus licheniformis; Brucella ovis; Brucella spp.; Campylobacter fetus ss. fetus; Campylobacter fetus ss. venerealis (Cfv); Enterobacteriaceae; Escherichia coli​​​​; Histophilus somni (Histophilus ovis); Histophilus spp.; Mortierella wolfii; Pseudomonas aeruginosa; Salmonella spp.; Staphylococcus spp.; Streptococcus spp.; Trueperella pyogenes; Identification of unknown bacteria Biochemical; Commercial identification kit; Cultural; Microscopic examination; Staining

in-house methods:

MMBAC-104 (The genital system - Microscopy and culture);

MMBAC-114 (Ram reproductive Tract - Sampling and culture); and 

MMBAC-115 (Culture of ram semen)

MMBAC-500 (Follow-up Methods and Other Tests)
Detection and identification of bacteriaFor production animals (ovine, bovine) and avian species Intestinal contents; Smears Clostridium perfringens epsilon toxin Counter immunoelectrophoresis (CIEP)

in-house method PMBAC-109 (Counterimmunoelectrophoresis test (C.I.E.P.) method)

Detection and identification of fungi and mouldsFor production animals (bovine, porcine, caprine), companion animals (canine, feline), wildlife, equine species and avian species Hair; Nails; Skin Aspergillus spp.; Microsporum canis; Microsporum distortum; Microsporum gypseum; Microsporum nanum; Trichophyton equinum; Trichophyton mentagrophytes; Trichophyton varerinacei; Trichophyton verrucosum; Identification of unknown fungi; Yeasts; Zygomycetes Cultural; Microscopic examination; Staining

in-house methods: 

MMBAC-120 (Skin scrapings, hair & nail (Fungal culture));

PMBAC-132 (Stain procedure – LPCB), and: 

PMBAC-118 (Stain procedure - Gram)

Detection and identification of other microorganismsFor production animals (bovine, ovine, caprine) Fluids; Tissues Mycoplasma spp. Cultural; Microscopic examination

in-house method MMBAC-117 (Culture methods for mycoplasma)

EMAI – MaP – Molecular Biology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Detection and identification of bacteria Bacterial isolates Identification of unknown bacteria PCR - 16S Universal in-house method PMMBAC-10 (16S rRNA PCR and Sequencing)
Detection and identification of bacteriaFor production animals (bovine, ovine, caprine, camelid), companion animals (canine, feline) and equine species Fluids; Genital specimens Campylobacter fetus PCR - Multiplex

in-house method PMMBAC-09

Detection and identification of bacteriaFor production animals (bovine, ovine, caprine, camelid) Bacterial isolates; Fluids; Smears; Tissues; Whole blood Bacillus anthracis PCR - Multiplex

in-house method PMMBAC-05

Detection and identification of bacteriaFor production animals (bovine, ovine, porcine, caprine, camelid), companion animals (canine, feline), equine species and avian species Bacterial isolates; Semen Actinobacillus seminis; Brucella ovis; Histophilus somni (Histophilus ovis) PCR - Multiplex

in-house methods PMMBAC-019, PMMBAC-07, PMMBAC-06 and PMMBAC-08

EMAI – MaP – Parasitology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Detection and identification of parasites and arthropodsFor production animals (bovine, ovine) Faeces Liver fluke Enzyme linked immunosorbent assay (ELISA)

in-house method WISER-125; manufacturers instruction (Bio -X Diagnostic Liver fluke antigen ELISA Kit) 

Detection and identification of parasites and arthropodsFor production animals (ruminants - bovine, ovine, caprine), camelids, equine species Faeces Dictyocaulus filarial larvae; Dictyocaulus viviparous larvae; Muellerius spp. larvae; Protostrongylus spp. larvae; Lungworm larvae Baermann technique in-house method MPAR-040
Strongylid egg count Faecal floatation

in-house method MPAR-042 based on Cornell-Wisconsin centrifugal floatation technique, Egwang and Slocombe 1982

Faeces; Intestinal contents Liver fluke; Stomach fluke Sedimentation

in-house method MPAR-002,  

in-house method PAM-26, Department of Food and Agriculture approved fluke egg sedimentation test (FEST) procedure 

Chabertia larvae; Cooperia larvae; Cyathostomin larvae; Haemonchus larvae; Oesophagostomum larvae; Ostertagia larvae; Strongyloides larvae; Strongylus larvae; Trichostrongylus larvae; Larval counts Cultural; Microscopic examination; Staining in-house method MPAR-041
Faeces; Intestinal contents; Smears Coccidia Microscopic examination

in-house method MPAR-023 (smear)

Fresh or preserved parasites Identification of unknown parasites Microscopic examination; Morphological identification

in-house method MPAR-030

Gastrointestinal tract specimens Identification of unknown parasites Microscopic examination; Morphological identification

in-house method MPAR-006

Detection and identification of parasites and arthropodsFor production animals (ruminants - bovine, ovine, caprine, porcine, ungulates), companion animals (feline), camelids, equine species Faeces; Intestinal contents Ascarid egg count; Coccidia oocyst count; Nematodirus egg count; Strongylid egg count; Trichuris egg count; Tapeworm egg count Microscopic examination

in-house method MPAR-001 - Modified McMaster method

EMAI – MaP – Serology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine) Plasma; Serum Brucella abortus antibody Enzyme linked immunosorbent assay (ELISA)

in-house method PMSER-120

Vaginal swabs in transport media Campylobacter fetus ss. venerealis (Cfv) IgA antibody Enzyme linked immunosorbent assay (ELISA)

in-house method PMSER-010

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine, ovine) Plasma; Serum Brucella abortus antibody Serum agglutination

in-house method PMSER-011

Salmonella enterica serovar Dublin antibody; Salmonella enterica serovar Typhimurium antibody Serum agglutination

in-house method PMSER-004

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine, ovine) Milk; Serum Liver fluke Enzyme linked immunosorbent assay (ELISA)

in-house method WISER-111, manufacturers instructions (IDEXX antibody ELISA test kit)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine, ovine, porcine, caprine) and companion animals (canine) Serum Brucella abortus antibody; Brucella melitensis antibody; Brucella suis antibody Rose Bengal plate

in-house method PMSER-013 (Rose Bengal rapid slide agglutination test)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ovine, bovine, caprine and porcine) and companion animals (canine) Serum Brucella melitensis antibody; Brucella ovis; Brucella suis antibody; Chlamydia spp.; Coxiella burnetii (Q-fever) antibody; Mycobacterium paratuberculosis (Johne’s disease) - Bovine; Mycobacterium paratuberculosis (Johne’s disease) - Caprine; Mycobacterium paratuberculosis (Johne’s disease) - Ovine Complement fixation test (CFT)

in-house method PMSER-001

Brucella abortus antibody Complement fixation test (CFT)

in-house method PMSER-008

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ovine, bovine, cervine and caprine) Plasma; Serum Mycobacterium paratuberculosis (Johne's disease) - Cervine; Mycobacterium paratuberculosis (Johne’s disease) - Bovine; Mycobacterium paratuberculosis (Johne’s disease) - Caprine; Mycobacterium paratuberculosis (Johne’s disease) - Ovine Enzyme linked immunosorbent assay (ELISA)

in-house method PMSER-012, manufacturer’s instructions -  ID Screen Paratuberculosis Indirect Screening Kit and ID Screen Paratuberculosis Indirect confirmation kit

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ovine, bovine, porcine) and companion animals (canine) Fluids; Serum Leptospira australis antibody; Leptospira canicola antibody; Leptospira copenhageni antibody; Leptospira grippotyphosa antibody; Leptospira hardjo antibody; Leptospira icterohaemoragiae antibody; Leptospira pomona antibody; Leptospira trassovi antibody; Leptospira zanini antibody Microscopic agglutination

in-house methods PMSER-007

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ovine, caprine, camelids) Plasma; Serum Mycobacterium paratuberculosis (Johne’s disease) - Caprine; Mycobacterium paratuberculosis (Johne’s disease) - Ovine Agar gel immunodiffusion (AGID)

in-house method PMSER-003

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (porcine) Plasma; Serum Mycoplasma hyopneumoniae Enzyme linked immunosorbent assay (ELISA) in-house method WISER-121, manufacturers instructions – HerdCheck test kit
Actinobacillus pleuropneumoniae Enzyme linked immunosorbent assay (ELISA) in-house method WISER-119, manufacturers instructions (IDEXX antibody ELISA test kit)
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ruminants) Plasma; Serum Coxiella burnetii (Q-fever) antibody Enzyme linked immunosorbent assay (ELISA)

in-house method WISER-124, manufacturers instructions (IDEXX antibody ELISA test kit)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals Serum Toxoplasma gondii antibody Latex agglutination

in-house method WISER-010; manufacturers instructions (Elitex Toxo kit)

EMAI – Veterinary Pathology Service

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Examination of biopsy material Tissues Histological examination Histopathology; Macroscopic examination; Microscopic examination; Special stains in-house methods: WIHIS-001 (Dewaxing and Rehydrating); WIHIS-002 (Dehydrating, Clearing and Cover-slipping); PMHIS-038 (Fixation and decalcification); PMHIS-013 (Alcian Blue stain technique); PMHIS-014 (Congo Red); PMHIS-015 (Rhodanine stain technique); PMHIS-016 (Giemsa Stain technique); PMHIS-017 (Gomori's Hexamine Silver stain technique); PMHIS-021 (Luxol Fast Blue); PMHIS-019 (Gram (modified) Stain Technique); PMHIS-022 (Masson's Trichrome Stain Technique); PMHIS-023 (Melanin Permangenate Bleach Stain technique); PMHIS-026 (Periodic Acid Schiff"s Stain Technique); PMHIS-028 (Perles prussian blue stain technique); PMHIS-030 (Toluidine Blue Stain Technique); PMHIS-032 (Von Kossa stain technique); PMHIS-033 (Warthin Starry stain technique); PMHIS-034 (Ziehl Neelsen stain technique); PMHIS-035 (Modified ZN technique); PMHIS-036 (Diff Quick Blood Smears); PMHIS-037 (Giemsa Blood Smears); PHHIS-039 (Sample Management Histology); PMHIS-043 (Processing Central Nervous Tissues); PMHIS-048 (Cell Culture Stain Technique); PMHIS-102 (Haematoxylin and Eosin stain technique)
Prion disease lesions Histopathology Australian and New Zealand Standard Diagnostic Procedure, Transmissible Spongiform Encephalopathies, August 2010, 
Necropsy servicesFor production animals, production avian species, wildlife, aquatic animals, equine species and avian species Body; Tissues Gross pathology and collection of sub-samples Anatomical pathology; Microscopic examination

in-house method

EMAI – Virology

ISO/IEC 17025 (2017)

Animal Health

Service Product Determinant Technique Procedure
Analysis for transmissible animal diseases under the European Union Directives for Animal Health Bovine reproductive material Bovine viral diarrhoea (BVD) Agar gel immunodiffusion (AGID); Enzyme linked immunosorbent assay (ELISA); PCR - Quantitative (qPCR) - Reverse transcription; Serum neutralisation in-house methods VLP015 (AGID), VLP168 (Ag capture ELISA), VLP023 (Ab ELISA), VLP165 (PCR)
Bovine reproductive material; Ovine reproductive material Bluetongue virus (BTV) Agar gel immunodiffusion (AGID); Enzyme linked immunosorbent assay (ELISA); PCR - Quantitative (qPCR) - Reverse transcription in-house methods VLP004 (AGID), VLP20 (ELISA), VLP190 (ELISA), VLP175 (PCR)
Epizootic haemorrhagic disease virus Agar gel immunodiffusion (AGID) in-house method VLP-008
Equine reproductive material Equine infectious anaemia Agar gel immunodiffusion (AGID) in-house method VLP009
Equine arteritis virus Serum neutralisation; Virus isolation in-house methods VLP106 (VNT), VLP095 (VI)
Ovine reproductive material Border disease Virus isolation in-house method VLP094
Detection and identification of viruses Allantoic fluid; Biological products; Buffy coat; Cerebrospinal fluid (CSF); Culture materials; Dust; Ear notch; Embryos; Faeces; Feathers; Fluids; Milk; Plasma; Semen; Serum; Swabs; Tissues; Whole blood Identification of unknown viruses Transmission electron microscopy (TEM)

in-house method VLP149 (Transmission electron microscopy by negative staining)

Semen; Serum; Swabs; Tissues; Whole blood Hendra virus PCR - Quantitative (qPCR)

in-house method VLP182

Porcine circovirus PCR - Quantitative (qPCR)

in-house method VLP188

Semen; Swabs; Tissues Bovine herpes virus type 1 PCR - Quantitative (qPCR)

in-house method VLP172

Semen; Tissues; Whole blood Equine arteritis virus PCR - Quantitative (qPCR)

in-house method VLP189

Serum; Whole blood Bovine ephemeral fever virus Cultural; Embryonated egg culture

in-house method VLP095

Swabs; Tissues White spot syndrome virus (WSSV) PCR - Quantitative (qPCR)

in-house method VLP161

Influenza A virus PCR - Quantitative (qPCR)

in-house method VLP155

Yellow head virus PCR - Quantitative (qPCR)

in-house method VLP162

Newcastle disease virus (NDV) PCR - Quantitative (qPCR)

in-house method VLP160

Equine influenza virus PCR - Quantitative (qPCR)

in-house method VLP167

Tissues Ostreid herpes virus 1 PCR - Quantitative (qPCR)

in-house method VLP179

Tissues; Whole blood Bovine ephemeral fever virus PCR - Quantitative (qPCR)

in-house method VLP183

Detection and identification of virusesFor aquatic animals Tissues Nodavirus PCR - Quantitative (qPCR)

in-house method VLP158

Detection and identification of virusesFor production animals (bovine, ovine, caprine) Culture materials Bluetongue virus (BTV); Bovine ephemeral fever virus; Bovine viral diarrhoea virus (BVDV); Border disease Immunoperoxidase staining (IPX)

in-house method VLP120

Detection and identification of virusesFor production animals (bovine, ruminants) Embryos; Semen; Serum; Whole blood Bluetongue virus (BTV); Pestivirus Cultural

in-house methods VLP093 and VLP094

Embryos; Semen; Tissues; Whole blood Aino virus PCR - Quantitative (qPCR)

in-house method VLP185

Detection and identification of virusesFor production animals (bovine, ruminants, camelids, cervids) Embryos; Semen; Tissues; Whole blood Bluetongue virus (BTV) PCR - Quantitative (qPCR)

in-house method VLP175

Detection and identification of virusesFor production animals (bovine, ruminants, camelids, porcine) Hair follicle; Semen; Serum; Swabs; Tissues; Whole blood Pestivirus PCR - Quantitative (qPCR)

in-house method VLP165

Detection and identification of virusesFor production animals (porcine) Semen; Serum; Swabs; Tissues; Whole blood Bungowannah virus PCR - Quantitative (qPCR)

in-house method VLP187

Detection and identification of virusesFor production animals (ruminants and camelids) Semen; Serum; Swabs; Tissues; Whole blood Epizootic haemorrhagic disease virus PCR - Quantitative (qPCR)

in-house method VLP186

Detection and identification of virusesFor production animals (ruminants) Embryos; Semen; Tissues; Whole blood Akabane virus PCR - Quantitative (qPCR)

in-house method VLP184

Detection and identification of virusesFor production animals, production avian species, laboratory animals, zoo animals, wildlife, aquatic animals, equine species and avian species Allantoic fluid; Embryos; Fluids; Semen; Serum; Tissues; Whole blood Identification of unknown viruses Cultural; Embryonated egg culture

in-house method VLP095

Immunoglobulin investigationsFor production animals (bovine) Serum Immunoglobulin G (IgG) Agar gel immunodiffusion (AGID)

in-house method VLP88

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitation Bulk milk Enzootic bovine leukosis virus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP029, manufacturers instructions (IDEXX, Svanova and Synbiotics kits)

Milk Enzootic bovine leukosis virus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP030, manufacturers instructions (IDEXX, Svanova and Synbiotics kits)

Plasma; Serum Equine influenza virus Haemagglutination inhibition

in-house method VLP173

Caprine arthritis encephalitis virus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP027

Enzootic bovine leukosis virus Agar gel immunodiffusion (AGID)

in-house method VLP7

Bovine viral diarrhoea virus (BVDV) Agar gel immunodiffusion (AGID)

in-house method VLP15

Equine infectious anaemia virus Agar gel immunodiffusion (AGID)

in-house method VLP9

Avian influenza Haemagglutination inhibition

in-house method VLP83

Avian influenza Agar gel immunodiffusion (AGID)

in-house method VLP2

Rabbit calicivirus antibody Enzyme linked immunosorbent assay (ELISA)

in-house method VLP022

Infectious bovine rhinotracheitis (IBR) Enzyme linked immunosorbent assay (ELISA)

in-house method VLP034, manufacturers instructions (IDEXX kit)

Bovine ephemeral fever virus Enzyme linked immunosorbent assay (ELISA)

in-house method MVIR-031

Newcastle disease virus (NDV) Haemagglutination inhibition

in-house method MVIR085

Hendra virus soluble G Enzyme linked immunosorbent assay (ELISA)

in-house method VLP033

Serum Infectious bovine rhinotracheitis (IBR) Serum neutralisation

in-house method VLP110

Fowl adenovirus Serum neutralisation

in-house method VLP108

Enzootic bovine leukosis virus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP028, manufacturers instructions (IDEXX, Svanova and Synbiotics kits)

Equine herpes virus type 1 Serum neutralisation

in-house method VLP107

Equine arteritis virus Serum neutralisation

in-house method VLP106

Tissues Rabbit calicivirus (rabbit haemorrhagic disease) Enzyme linked immunosorbent assay (ELISA)

in-house method VLP026

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor equine species Fluids; Plasma; Serum West Nile virus IgM - Equine Enzyme linked immunosorbent assay (ELISA)

in-house method VLP-193, Manufacturers instructions (IDVet kit) 

Serum Ross River virus Serum neutralisation

in-house method VLP115

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine) Plasma; Serum Parainfluenza virus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP035, manufacturers instructions (IDEXX kit)

Respiratory syncytial virus (RSV) Enzyme linked immunosorbent assay (ELISA)

in-house method VLP036, manufacturers instructions (svanova kit)

Serum Ephemeral fever virus Serum neutralisation

in-house method VLP104

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine, ruminants and camelids) Plasma; Serum Bluetongue virus (BTV) Agar gel immunodiffusion (AGID)

in-house method VLP4

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine, ruminants) Milk; Plasma; Serum Pestivirus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP023, manufacturers instructions (IDEXX Ab kit)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (bovine, ruminants, camelids) Clotted blood; Tissues Bluetongue virus (BTV) Enzyme linked immunosorbent assay (ELISA) - Antigen capture

in-house method VLP037

Plasma; Serum Bluetongue virus (BTV) Enzyme linked immunosorbent assay (ELISA)

in-house method VLP20, VLP190

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ovine, ruminants, camelids) Plasma; Serum Epizootic haemorrhagic disease virus Agar gel immunodiffusion (AGID) in-house method VLP8
Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (porcine) and zoo animals Serum Encephalomyocarditis Serum neutralisation

in-house method VLP103

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (porcine), equine species and avian species Plasma; Serum Influenza A virus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP019; manufacturers instructions (IDEXX and IDVet test kits)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ruminants) Plasma; Serum Akabane virus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP018

Serum Pestivirus Serum neutralisation

in-house method VLP113

Akabane virus Serum neutralisation

in-house method VLP100

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ruminants, bovine) Serum Aino virus Serum neutralisation

in-house method VLP099

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ruminants, camelids) Clotted blood; Hair; Plasma; Serum; Skin; Tissues Pestivirus Enzyme linked immunosorbent assay (ELISA) - Antigen capture

in-house method VLP168, manufacturers instructions (IDEXX kit)

Microbiology - Serology of infection - Microbial antibody and/or antigen detection and/or quantitationFor production animals (ruminants, ovine) Plasma; Serum Simbu virus Enzyme linked immunosorbent assay (ELISA)

in-house method VLP024, manufacturers instructions (IDEXX kit)

ISO/IEC 17025 (2017)

Healthcare, Pharmaceutical and Media Products

Service Product Determinant Technique Procedure
Testing for sterility and freedom from contamination of biological materials intended for veterinary use - Innocuity Raw materials of animal origin Bovine viral diarrhoea virus (BVDV); Infectious bovine rhinotracheitis virus; Parainfluenza virus type 3; Porcine parvovirus Virus isolation in-house method VLP095 Appendix 4 & 5

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